NSC 80467, a selective DNA damaging agent, functions by inhibiting survivin. Specifically, it preferentially targets DNA synthesis, leading to the induction of two distinct markers of DNA damage, namely γH2AX and pKAP1.

NSC 80467 has a similar spectrum of activity against the NCI-60 cell line panel, suppresses survivin expression, shows COMPARE correlation with DNA damaging agents, preferentially inhibits DNA synthesis, and induces a DNA damage response characterized by enhanced γH2AX and pKAP1 at concentrations lower than those capable of inhibiting survivin expression[1]. NSC 80467 (200 or 800 nM; 24 hours) inhibits surviving[1]. Western Blot Analysis[1]Cell Line: PC3 cells Concentration: 200 or 800 nM Incubation Time: 24 hours Result: Associated with inhibition of survivin expression.