validation

Methyl Nonadecanoate (Nonadecanoic Acid methyl ester)是一种内标物，能够用于生物柴油中脂肪酸甲酯含量的测定。

FKBP12 PROTAC dTAG-13是一种PROTAC和选择性降解剂，通过接合FKBP12 F36V和CRBN从而降解 FKBP12 F36V，可用于药物发现过程中的靶标验证。

GlyRS-IN-1 是在专利 WO 2017066459 A1 中披露的一种 glycyl-tRNA 合成酶（GlyRS）抑制剂，其通过干扰氨酰-tRNA 的生物合成而抑制蛋白质合成；同时，GlyRS-IN-1 还可抑制细菌生长，因此成为研究翻译机制、抗菌靶点验证及开发新型耐药细菌抗生素的重要化学探针。

PROTAC FKBP Degrader-3 是一种双功能 PROTAC 分子，由 FKBP 配体结合基团、经合理设计的连接臂以及 VHL E3 泛素连接酶结合基团组成，可作为高效的 FKBP 降解剂，用于选择性蛋白降解研究及 FKBP 作为治疗靶点的功能验证。

JXL069 是一种最初由加州大学洛杉矶分校（University of California, Los Angeles，UCLA）开发的实验性药物，其作为一种高效且高度选择性的线粒体丙酮酸转运载体（mitochondrial pyruvate carrier，MPC）抑制剂，可有效阻断丙酮酸向线粒体内的受控转运过程，从而重塑细胞能量代谢状态。该化合物在动物模型研究中通过明确的作用机制验证，显示出作为代谢调节剂在雄激素性脱发治疗领域中的潜在应用价值和良好的临床前研究前景。

Desmethyl ranolazine is a metabolite of the antianginal agent ranolazine .1It is formed from ranolazine by the cytochrome P450 (CYP) isoform CYP3A. 1.Wang, Y., Chen, X., Sun, Z., et al.Development and validation of a sensitive LC-MS/MS assay for simultaneous quantitation of ranolazine and its three metabolites in human plasmaJ. Chromatogr. B889-89010-16(2012)

Palonosetron N-oxide is a metabolite of the serotonin (5-HT) receptor subtype 5-HT3antagonist palonosetron .1It is also a potential impurity in palonosetron preparations.2Palonosetron N-oxide is a degradation product formed by exposure to oxidative stress. 1.Stoltz, R.A., Cyong, J.-C., Shah, A., et al.Pharmacokinetic and safety evaluation of palonosetron, a 5-hydroxytryptamine-3 receptor antagonist, in U.S. and Japanese healthy subjectsJ. Clin. Pharmacol.44(5)520-531(2004) 2.Vishnu Murthy, M., Srinivas, K., Kumar, R., et al.Development and validation of a stability-indicating LC method for determining palonosetron hydrochloride, its related compounds and degradation products using naphthalethyl stationary phaseJ. Pharm. Biomed. Anal.56(2)429-435(2011)

5,6-dimethyl-2-Thiouracil is a heterocyclic building block that has been used in the synthesis of anti-HIV-1 pyrimidinones.1 It has also been used as an internal standard for the quantification of thyreostats, including 2-thiouracil, in bovine plasma.2 |1. Navrotskii, M.B. Synthesis and anti-HIV-1 activity of new 2-[(2-phthalimidoethyl)thio]-4(3H)-pyrimidinone derivatives. Pharm. Chem. J. 39(9), 466-467 (2005).|2. Schmidt, K.S. In-house validation and factorial effect analysis of a liquid chromatography-tandem mass spectrometry method for the determination of thyreostats in bovine blood plasma. Anal. Bioanal. Chem. 406(3), 735-743 (2014).

O-desmethyl Brinzolamide is an active metabolite of the carbonic anhydrase (CA) inhibitor brinzolamide .1,2It inhibits CAII and CAIV (IC50s = 0.136 and 165 nM, respectively).1 1.Huang, Q., Rui, E.Y., Cobbs, M., et al.Design, synthesis, and evaluation of NO-donor containing carbonic anhydrase inhibitors to lower intraocular pressureJ. Med. Chem.58(6)2821-2833(2015) 2.Lo Faro, A.F., Tini, A., Gottardi, M., et al.Development and validation of a fast ultra-high-performance liquid chromatography tandem mass spectrometry method for determining carbonic anhydrase inhibitors and their metabolites in urine and hairDrug Test Anal.13(8)1552-1560(2021)

CAY10781 是一种神经纤毛蛋白-1 (NRP-1) 和 VEGF-A.1 之间蛋白质-蛋白质相互作用的抑制剂。当使用浓度为 12.5 μM 时，它可抑制 43% 的相互作用。 当以相同浓度使用时，CAY10781 还抑制 VEGF-A 诱导的儿茶酚胺 A 分化 (CAD) 细胞中 VEGFR2 的磷酸化。

Sparstolonin B 是一种选择性 TLR2 和 TLR4 拮抗剂，是一种是一种从 Sparganium stoloniferum 和 Scirpus yagara 的块茎中分离出的异香豆素化合物，具有抗 HIV 、抗癌、抗肿瘤和抗炎活性，抑制选择性Toll样受体，抑制游离脂肪酸棕榈酸酯诱导的软骨细胞炎症并减轻肥胖小鼠的创伤后关节炎，抑制脂多糖诱导的 3T3-L1 脂肪细胞炎症，可用于研究验证和乳腺癌。

PSB-KD477 is an Agonist of the Cannabinoid-Activated Orphan G-Protein-Coupled Receptor GPR18. PSB-KD477 displayed significantly higher potency and efficacy than THC, determined in a GPR18-dependent β-arrestin recruitment assay, and were found to be selective versus the CB-sensitive receptors CB1, CB2, and GPR55. Structure-activity relationships were steep, and indole substitution was crucial for biological activity. These first selective agonists, which are structurally distinct from the lipidic agonist(s), will allow target validation studies and may eventually contribute to the deorphanization of GPR18.

Ritlecitinib, also known as PF-06651600, is a potent and selective JAK3 inhibitor. PF-06651600 is a potent and low clearance compound with demonstrated in vivo efficacy. The favorable efficacy and safety profile of this JAK3-specific inhibitor PF-06651600 led to its evaluation in several human clinical studies. JAK3 was among the first of the JAKs targeted for therapeutic intervention due to the strong validation provided by human SCID patients displaying JAK3 deficiencies.

PFI-6 is a selective and non-cytotoxic probe targeting the YEATS domain of MLLT1 (ENL/YEATS1) and MLLT3 (AF9/YEATS3), with IC50 values of 140 nM for MLLT1 and 160 nM for MLLT3. It serves as a molecular tool to investigate the role of MLLT1/3 in diseases and genome validation studies [1].

4,4'-Bipyridyl (Standard)是 4,4‘-Bipyridyl 的认证参考标准品，用于定量分析、分析校准、质量控制及生物化学实验，广泛应用于需要精确化合物鉴定、方法学验证及可重复理化基准的生命科学和化学研究。

Coenzyme Q10 (Standard)是Coenzyme Q10 的认证参考标准品，设计用于定量分析、分析方法学验证、质量控制流程以及生物化学实验研究。Coenzyme Q10（CoQ10）是一种天然来源的分子，由藤仓型真菌产生，在生物体系中作为线粒体呼吸链中的关键电子载体，对细胞能量代谢及氧化还原稳态的维持具有至关重要的作用。

Chenodeoxycholic acid (Standard)是一种用于研究和定量分析的分析参考标准，代表一种疏水性初级胆汁酸，可激活参与胆固醇稳态、胆汁酸代谢及脂质调控的核型 farnesoid X 受体信号通路，广泛用于生化验证、代谢研究及分析方法开发。

meso-Erythritol (Standard)是用于科研和分析验证的 meso-Erythritol 分析参考标准，属于天然存在的糖醇，广泛存在于梨、西瓜等水果中，甜度约为蔗糖的 60–80%。作为获批的低热量甜味剂，meso-Erythritol 在代谢与营养学研究中得到广泛应用。

Excavatin G 是新型香豆素类化合物，可从黄皮 (Clausena excavata) 中获得。虽然黄皮常用于杀虫和缓解胃痛，但 Excavatin G 的特定活性和功能仍需进一步研究验证。

11β,13-Dihydrotaraxinic acid 是一种天然存在的小分子代谢物，被鉴定为适用于生命科学和生物化学研究的生物活性化合物。该化合物主要作为参考品或研究级材料，用于天然产物化学、代谢通路分析、生物活性筛选以及药理学、生物化学或分子生物学领域中的实验验证研究，尤其适用于需要结构明确的植物来源化合物的研究体系。