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FGFR2 Protein, Human, Recombinant (alpha(IIIb), His & Avi), Biotinylated

产品编号 TMPY-06321
FGFR2 Protein, Human, Recombinant (alpha(IIIb), His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 42.88 kDa and the accession number is NP_075259.4.
规格价格库存数量
20 μg¥ 3,8205日内发货
100 μg¥ 8,8405日内发货
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生物活性

生物活性
Immobilized Recombinant Human FGF10 / KGF2 Protein at 2 μg/mL (100 μL/well) can bind Recombinant Human FGFR2 Protein (alpha IIIb, ECD, His & AVI Tag), Biotinylated , the EC50 is 60-180 ng/mL.
产品描述
FGFR2 Protein, Human, Recombinant (alpha(IIIb), His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 42.88 kDa and the accession number is NP_075259.4.
种属
Human
表达系统
HEK293 Cells
标签C-His-Avi
蛋白编号NP_075259.4
别名
ECT1,CEK3,JWS,TK14,K-SAM,CD332,TK25,KGFR,fibroblast growth factor receptor 2,BFR-1,BEK,FGFR2 α(IIIb),CFD1,BBDS
蛋白构建
A DNA sequence encoding the Human FGFR2 (NP_075259.4)(Met1-Glu378) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Arg 22
蛋白纯度
≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
分子量42.88 kDa (predicted); 70 kDa (reducing conditions)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
FGFR2, also known as CD332, belongs to the fibroblast growth factor receptor subfamily where amino acid sequence is highly conserved between members and throughout evolution. FGFR2 acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. It is required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. FGFR2 plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. It also promotes cell proliferation in keratinocytes and imature osteoblasts, but promotes apoptosis in differentiated osteoblasts. FGFR2 signaling is down-regulated by ubiquitination, internalization and degradation. Mutations that lead to constitutive kinase activation or impair normal CD332 maturation, internalization and degradation lead to aberrant signaling. Over-expressed FGFR2 promotes activation of STAT1. Defects in CD3322 are the cause of Crouzon syndrome, Jackson-Weiss syndrome, Apert syndrome, Pfeiffer syndrome, Beare-Stevenson cutis gyrata syndrome, familial scaphocephaly syndrome, lacrimo-auriculo-dento-digital syndrome and Antley-Bixler syndrome without genital anomalies or disordered steroidogenesis.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

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