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DNA PolymeraseBeta Protein, Human, Recombinant (His)

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货号 TMPJ-00792

别名 POLB, DNA Polymeraseβ, DNA Polymerase β, DNA Polymerase Beta

Human DNA polymerase β is constitutively expressed in cells. It fills in gaps in DNA that are formed following base excision repair. Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. It conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. The activity cannot be affected by Aphidicolin, which is an inhibitor of DNA polymerase β.

DNA PolymeraseBeta Protein, Human, Recombinant (His)
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DNA PolymeraseBeta Protein, Human, Recombinant (His)

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Rating icon 还可以
货号 TMPJ-00792 别名 POLB, DNA Polymeraseβ, DNA Polymerase β, DNA Polymerase Beta

Human DNA polymerase β is constitutively expressed in cells. It fills in gaps in DNA that are formed following base excision repair. Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. It conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. The activity cannot be affected by Aphidicolin, which is an inhibitor of DNA polymerase β.

规格价格库存数量
5 μg
¥ 492
6-8日内发货
10 μg
¥ 820
6-8日内发货
20 μg
¥ 1,290
5日内发货
50 μg
¥ 2,480
5日内发货
100 μg
¥ 3,980
5日内发货
200 μg
¥ 6,420
5日内发货
500 μg
¥ 12,100
5日内发货
1 mg
¥ 17,300
5日内发货
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产品介绍

生物活性
产品描述
Human DNA polymerase β is constitutively expressed in cells. It fills in gaps in DNA that are formed following base excision repair. Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. It conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. The activity cannot be affected by Aphidicolin, which is an inhibitor of DNA polymerase β.
生物活性
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
研究背景
Human DNA polymerase β is constitutively expressed in cells. It fills in gaps in DNA that are formed following base excision repair. Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. It conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. The activity cannot be affected by Aphidicolin, which is an inhibitor of DNA polymerase β.
种属
Human
表达系统
E. coli
标签C-6xHis
蛋白编号P06746
氨基酸序列Ser2-Glu335
蛋白构建Ser2-Glu335
蛋白纯度
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
缓冲液Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 1 mM EDTA, 50% Glycerol, pH 7.8.
别名POLB, DNA Polymeraseβ, DNA Polymerase β, DNA Polymerase Beta
内毒素< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
化学信息
分子量39 KDa (reducing condition)
储存&溶解度
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
存储Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

计算器

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  • 重组蛋白稀释 计算器
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剂量转换

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技术支持

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