TrxR-IN-3 (Compound 2c) is a effective TrxR inhibitor. TrxR-IN-3 shows strong antiproliferative activities against five human cancer cell lines, especially against breast tumor cells. TrxR-IN-3 increases ROS levels and leads to significant apoptosis by regulating the expression of apoptosis-related proteins in the breast cancer cells. TrxR-IN-3 also triggers the formation of autophagosomes and autolysosomes by promoting the expression of LC3-II and Beclin-1 and reducing the expression of LC3-I and p62 proteins [1].