fluorescent probes

Fluorescein Sodium (VST-1001) 是一种具有代表性的绿色荧光示踪剂，广泛用于医学、生物应用和肿瘤感染组织的示踪剂，也可作为实用绿色荧光探针的支架。

3-Cyano-7-hydroxycoumarin (3-Cyanoumbelliferone) 是一种巨噬细胞游走抑制因子 (MIF) 互变异构酶抑制剂，Ki 为 2.9 μM。3-Cyano-7-hydroxycoumarin 属于香豆素衍生物，可被用作分子探针和荧光染料。

2-Di-1-ASP 是单苯乙烯基染料，对 G-四链体和双链 DNA 具有选择性。它普遍用作双链 DNA 的线粒体染色和凹槽结合荧光探针。

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3 and Fluo-4 are most commonly used among the visible light-excitable calcium indicators. Fluo-4, pentaammonium salt is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels.

ZnAF-1F 是一种高效荧光团，Kd值为 2.2 nM，可用于作为细胞内 Zn2+ 的荧光探针。其激发波长为 489 nm，发射波长为 514 nm。

BODIPY R6G methyl ester (化合物 5′-1) 属于BODIPY家族的荧光染料，以其良好的光稳定性、高灵敏度和对pH值的非敏感性而受到青睐。这种化合物广泛应用于多个研究领域，包括光动力学、pH探测、离子识别、光催化、蛋白质标记、细胞成像及蛋白质组学分析等。

ICG-OSu, also known as ICG NHS ester, is a near-infrared fluorescent agent commonly utilized for the development of in vivo imaging probes due to its amine-reactive properties.

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3 and Fluo-4 are most commonly used among the visible light-excitable calcium indicators. Fluo-4, pentasodium salt is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels.

Fluo-8,AM is one of the most commonly used fluorescent probes to detect intracellular calcium concentration.Fluo-8, which penetrates the cell membrane and enters the cell, is cut by the intracellular esterase to form fluO-8, and thus is trapped in the cel

(±)-ANAP hydrochloride is the amino acid analog of prodan. It can be used as a fluorescent probes and enhance environmental sensitivity.

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3 and Fluo-4 are most commonly used among the visible light-excitable calcium indicators. Fluo-4, pentapotassium salt is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels.

BODIPY-FL (BDP FL acid) 是一种广谱且有效的荧光染料，可用于标记探针或引物，是一种基于荧光猝灭的定量检测特定 DNA RNA 的化合物。BODIPY-FL 标记的单萜可用于检测革兰氏阳性和革兰氏阴性细菌的特征和致病真菌。

(±)-ANAP is the amino acid analog of prodan. It can be used as a fluorescent probes and enhance environmental sensitivity.

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3, pentaammonium salt is most commonly used among the visible light-excitable calcium indicators.

ROX NHS酯，6-异构体，是一种具有高荧光和光稳定性的罗丹明染料，适用于各种应用。ROX标记的寡核苷酸探针常用于qPCR，[qPCR仪器具有ROX通道]。这种活性染料用于标记肽、蛋白质和氨基寡核苷酸中的氨基，且为纯单一异构体。

Quin-2 is a high-affinity fluorescent calcium indicator (Kd = 115 nM for calcium). It displays high selectivity for calcium, as it is not affected by sodium gradients, membrane potential, or intracellular pH. High affinity probes like quin-2 are ideal for monitoring low levels of calcium, as are found in resting cells. Loadings of up to 2 mM quin-2 are without serious toxic effects, so quin-2 may be used to buffer intracellular calcium transients. Excitation/emission maxima for quin-2 are 339 and 492 nm, respectively.

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3, pentasodium salt is most commonly used among the visible light-excitable calcium indicators.

HKOCl-4 (BXY2142) is a rhodol-based yellow fluorescent probe that demonstrates exceptional sensitivity and selectivity in detecting hypochlorous acid. Its absorption wavelength is significantly longer than that of fluorescein-based probes, and it exhibits better pH stability. Notably, HKOCl-4 exhibits excitation at 530 nm and emission at 557 nm.

5-Nitro BAPTA tetramethyl ester是一种钙螯合剂，用于双光子探针合成，实现细胞内钙离子实时成像，可检测肝组织内100-300 μm深度的钙波，持续1100-4000 s，并可与2-Me取代TM荧光化合物结合，制成红色荧光探针。

PM-1, a Thioflavin-T (ThT) derivative, is a small, highly specific fluorescent dye for plasma membrane (PM) imaging in living and fixed cells. It integrates directly into the cell membrane and retains its presence on the PM for about 15 hours. PM-1 can be combined with protein labeling probes to investigate ectodomain shedding and endocytosis of cell surface proteins [1].

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3, pentapotassium salt is most commonly used among the visible light-excitable calcium indicators.