Propidium Iodide (PI) 是一种可用于细胞染色的红色荧光染料,适用于荧光显微镜、共聚焦显微镜、流式细胞仪以及荧光计分析。在水溶液中,Propidium Iodide 的 Ex Em 为 493 636 nm;与核酸结合后,PI-核酸的 Ex Em 变为 535 617 nm,同时荧光信号增强 20-30 倍。
Phloxine B is a red dye, the USP grade of which is used as a color additive for food, drugs, and cosmetics. It has also been used as an alternative to Gram staining, to differentiate between Gram-negative and Gram-positive bacteria. At 100 μg/ml, it can inhibit methicillin-resistant S. aureus growth in vitro. Phloxine B has also been shown to modulate channel activity of wild-type cystic fibrosis transmembrane conductance regulator chloride channels (CFTR; Ki = 38 μM) and mutant delta F508 CFTR (Ki = 33 μM).
Alcian blue is a copper-containing dye used as a gelling agent for lubricants, for staining of bacteria and for the dyeing of histiocytes and fibroblasts in vivo.
C.I. 74240 is a copper-containing dye used as a gelling agent for lubricants, for staining of bacteria and for the dyeing of histiocytes and fibroblasts in vivo.
Cy5-DBCO (DBCO-Sulfo-Cy5) is a near-infrared (NIR) red fluorescent dye (λabs: 646 nm, λem: 670 nm), not suitable for staining intracellular components of permeabilized cells due to potential high background.
1) Non-toxicity: The unique oiliness and high molecular weight characteristics of CelRed prevent it from penetrating cell membranes into cells. Ames test results also show that the mutagenicity of CelRed dye is much less than that of EB. 2) High sensitivi
Jenner's Stain, a dye utilized in microscopy, is employed for staining blood smears, chromosome staining via the C-banding technique, and routine blood examinations, including malarial staining [1].
1) Non-toxicity: belong to flower-stem dye, easy to biodegrade, no carcinogenic toxicity. 2) High sensitivity: at least 100pg ssDNA or RNA can be detected, which is 25 ~ 100 times higher than EB staining. 3) High SIGNal-to-noise ratio: the sample fluorescence signal is strong, while the background signal is low. 4) Simple operation: like EB, the dye does not degrade in the prefabricated gel and electrophoresis process;The dyeing process after electrophoresis takes only 30 minutes without decoloring or washing, and can be observed directly with ultraviolet gel transmission or visible light transmission.
TMB monosulfate (BM blue monosulfate) is a chromogenic substrate used in staining procedures in immunohistochemistry and a visualizing reagent used in ELISA.
Biebrich scarlet is a scarlet dye, it has been used in Masson’s trichrome stain for the staining of collagen. It has also been used for the staining of eosinophil.
Nile Blue A (Nile blue sulfate) is used as the differentiation of melanins and lipofuscins. It is also useful for staining fats and the preparation of an amperometric glucose sensor.