BODIPY 500/510 C1, C12, a derivative of the robust BODIPY dye family, exhibits strong ultraviolet absorption and emits fluorescence with a high quantum yield at a sharp peak. This dye's effectiveness is minimally affected by environmental factors such as polarity or pH, and it maintains stability under various physiological conditions. BODIPY 500/510 C1, C12 has the unique ability to penetrate cell membranes and specifically target polar lipids within cells, effectively staining lipid droplets. This property makes it suitable for labeling both live and fixed cells [1]. It has a maximum excitation/emission wavelength of 500/510 nm [1]. For optimal preservation, it should be protected from light and stored at -20°C.

BODIPY 500/510 C1, C12 使用方法：1. 工作液的配制：制备储存液，使用247 μL无水DMSO稀释1mg BODIPY 500/510 C1, C12制备10 mM储备液，储存于-20℃或-80℃避光保存。使用预热的无血清细胞培养基或PBS稀释储存液，配制1-10 μM工作液，现用现配。2. 悬浮细胞染色：离心收集细胞，PBS洗涤两次（每次5 min），细胞密度为1×10^6/mL。加入1 mL BODIPY 500/510 C1, C12工作液，室温孵育5-30 min。400 g离心3-4 min后弃去上清，PBS洗涤两次（每次5 min）。用1 mL无血清培养基或PBS重悬细胞，使用荧光显微镜或流式细胞仪观察。3. 贴壁细胞染色：将贴壁细胞培养于无菌盖玻片上，从培养基中移出盖玻片，吸除多余培养基，加入100 μL染料工作液，轻轻晃动使其完全覆盖细胞，孵育5-30 min。吸去染料工作液，用培养基洗2-3次（每次5 min），使用荧光显微镜或流式细胞仪观察。保存条件：-20℃避光保存一年。注意事项：调整工作液浓度与孵育时间，实验建议进行阳性对照，将对照组细胞与30 μM油酸孵育8 h后进行后续实验。本产品仅限专业人员科学研究用，不得用于临床诊断或治疗，不得用于食品或药品。为保护安全和健康，请穿实验服并戴一次性手套操作。