5-Nitro BAPTA, a calcium chelator, combined with 2-Me-substituted TM (as a fluorescent moiety), forms a red fluorescent probe (CaTM-2 AM) for imaging cytoplasmic Ca 2+ in cultured living cells. It serves as a building block for synthesizing Ca 2+ specific chelators, Ca 2+ buffers, and fluorescent Ca 2+ indicators [1] [2].