(-)-Epigallocatechin Gallate (EGCG) is a phenolic antioxidant polyphenol flavonoid found in plants such as green and black tea, which inhibits telomerase and DNA methyltransferase, blocks the activation of EGF receptors and HER-2 receptors, inhibits cellular oxidation, and prevents free radical damage to cells.

方法：人甲状腺癌细胞 TT、TPC-1 和 ARO 用 (-)-Epigallocatechin Gallate (10-200 µM) 处理 24 h，使用 MTS assay 检测细胞活力。
结果：(-)-Epigallocatechin Gallate 以剂量依赖的方式抑制 TT、TPC-1 和 ARO 细胞的活力。[1]
方法：新生儿人类真皮成纤维细胞 nHDFs 用 (-)-Epigallocatechin Gallate (100-800 µmol/L) 处理 24 h，使用 Annexin V-FITC assay 检测细胞凋亡。
结果：在低于 200 µmol/L (-)-Epigallocatechin Gallate 的剂量下，既没有观察到细胞凋亡，也没有观察到坏死。然而，用更高浓度的 (-)-Epigallocatechin Gallate 处理 nHDFs 导致晚期细胞凋亡和坏死。[2]

方法：为研究对急性损伤的保护作用，将 (-)-Epigallocatechin Gallate (200 mg/kg) 灌胃给药给 ICR 小鼠，每天一次，持续一周，之后腹腔注射 LPS (15 mg/kg) 诱导急性损伤。
结果：(-)-Epigallocatechin Gallate 可以保护小鼠免受 LPS 诱导的急性应激，同时稳定肠道微生物，改善鞘脂代谢，抑制有害代谢产物的含量。[3]

LoVo, SW480, HCT-8, and HT-29 cells are seeded in 96-well plates at a concentration of 5×103?cells; each cell line is totally seeded in the 12 wells. Complete medium is added to the wells, up to 200 μL; the medium contains 0 μg/mL, 10 μg/mL, 20 μg/mL, and 35 μg/mL of epigallocatechol gallate. The inhibition rate=[1 - (absorbance of Epigallocatechol Gallate group - absorbance of control group)/(absorbance of control group - absorbance of blank control group)] × 100.