Naproxen sodium (Anaprox) is a COX inhibitor for COX-1 and COX-2 with analgesic and antipyretic properties.

COX-1:8.7 μM, COX-2:5.2 μM

在Carrageenan诱导关节炎的痛觉缺失大鼠模型中（IC50=27 μM）和酵母性发热大鼠模型中（IC50=40 μM）, Naproxen能够产生抑制作用.在大鼠中,Naproxen能够抑制脂多糖诱导的PGE2（IC50=12.8 μM）和TXB2（IC50=5.9 μM）的产生.

在HCA-7结肠癌细胞中（IC50=1.45 mM）,高浓度的Naproxen能够诱导细胞凋亡。在细胞内,Naproxen能够抑制COX-1（IC50=2.2 μg/mL）和 COX-2（IC50=1.3 μg/mL）。

COX-1 and COX-2 activities in intact cells: For the determination of COX-1 and COX-2 inhibition, bovine aortic endothelial cells (BAEC) are incubated for 30 minutes with Naproxen (0.1 ng/mL to 1 mg/mL), and cultured J774.2 macrophages are treated with endotoxin at 1 μg/mL for 12 hours to induce COX-2 followed by incubated for 30 minutes with Naproxen (0.1 ng/mL to 1 mg/mL), respectively. Arachidonic acid (30 μM) is then added, and the cells are incubated for a further 15 minutes at 37 °C. The medium is then removed, and radioimmunoassay is used to measure the formation of 6-keto-PGF1α, PGE2, thromboxane B2, or PGF2α for the assessment of IC50 for COX-1 and COX-2.

Cells are exposed to Naproxen for 24 and 48 hours, respectively. At the end of incubation, cells are harvested by trypsinization, stained with trypan blue solution (0.04% wt/vol) and counted in a Neubauer haemocytometer chamber for the determination of cell viability.(Only for Reference)