Triptolide (PG490) belongs to the tricyclic diterpenoid group of natural products and is an inhibitor of NF-κB activation. Triptolide exhibits immunosuppressive, anti-rheumatic, anti-inflammatory, anti-proliferative and anti-tumor activities.

MDM2-p53:47-73 nM (IC50)

方法：神经母细胞瘤细胞 BE(2)-C 用 Triptolide (5-100 nM) 处理 24 h，使用 CCK-8 Assay 检测细胞活力。
结果：在 BE(2)-C 细胞中观察到对 Triptolide 的浓度依赖性反应。在 50 nM 的 Triptolide 下，细胞活力显著降低至 50%。[1]
方法：乳腺癌细胞 MCF-7 用 Triptolide (10-50 nmol/L) 处理 24 h，使用 Western Blot 方法检测靶点蛋白表达水平。
结果：Triptolide 以剂量依赖的方式降低 MCF-7 细胞中 ERα 的信使核糖核酸水平和蛋白质水平。[2]

方法：为检测体内抗肿瘤活性，将 Triptolide (0.4 mg/kg) 灌胃给药给携带乳腺癌肿瘤 MCF-7 的 BALB/c-nu+/nu+ 小鼠，每天一次，持续三周。
结果：Triptolide 在小鼠模型中抑制 MCF-7 细胞异种移植物的生长。[2]
方法：为研究对溃疡性结肠炎 (UC) 的作用，将 Triptolide (0.1-0.4 mg/kg) 口服给药给 DSS 诱导 UC 的小鼠，每天一次，持续七天。
结果：Triptolide 对 UC 小鼠具有抗炎和治疗作用。[3]

Triptolide is dissolved in DMSO (1 mg/mL) and stored, and then diluted with RPMI 1640 medium before use[3]. The viability of differentiated PC12 cells treated with different concentrations of Triptolide. After differentiated PC12 cells are cultured on 96-well plates with RPMI 1640 medium for stabilization, differentiated PC12 cells are incubated with different concentrations of Triptolide (0.01, 0.1, and 1 nM) for 24 hours. The concentrations in this study are chosen. Then cell viability is determined by the MTT assay. Each condition and experiment is repeated three times[3].