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Paclitaxel (Taxol) 属于天然产物,是一种微管聚合物稳定剂。Paclitaxel 具有抗肿瘤活性;通过诱导有丝分裂停滞、细胞凋亡、细胞自噬等,导致细胞死亡。
Paclitaxel (Taxol) 属于天然产物,是一种微管聚合物稳定剂。Paclitaxel 具有抗肿瘤活性;通过诱导有丝分裂停滞、细胞凋亡、细胞自噬等,导致细胞死亡。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
1 mg | ¥ 128 | 现货 | |
5 mg | ¥ 253 | 现货 | |
10 mg | ¥ 355 | 现货 | |
25 mg | ¥ 530 | 现货 | |
50 mg | ¥ 745 | 现货 | |
100 mg | ¥ 1,230 | 现货 | |
500 mg | ¥ 2,930 | 现货 | |
1 mL x 10 mM (in DMSO) | ¥ 461 | 现货 |
产品描述 | Paclitaxel (Taxol) is a natural product and a microtubule polymer stabilizer. Paclitaxel has anti-tumor activity and causes cell death by inducing mitotic arrest, apoptosis, and cell autophagy. |
靶点活性 | Microtubule:4 nM |
体外活性 | 方法:人 T 细胞淋巴细胞白血病细胞 CCRF-HSB-2 用 Paclitaxel (0.01-1 µM) 处理 48 h,使用 MTT 方法检测细胞生长抑制情况。 结果:Paclitaxel 剂量依赖性地抑制 CCRF-HSB-2 细胞生长,IC50 为 0.25 µM。[1] 方法:人胃癌细胞 AGS 用 Paclitaxel (10-160 nM) 处理 24-48 h,使用 Western Blot 方法检测靶点蛋白表达水平。 结果:Paclitaxel 诱导凋亡相关蛋白 cleaved caspase-3 和 cleaved PARP 表达上调。[2] 方法:犬乳腺肿瘤细胞 CHMm 用 Paclitaxel (0.01-1 μM) 处理 24 h,使用 Flow Cytometry 方法检测细胞凋亡情况。 结果:Paclitaxel 剂量依赖性诱导 CHMm 细胞凋亡。[3] |
体内活性 | 方法:为研究低剂量 Paclitaxel 对肿瘤侵袭的影响,将 Paclitaxel (2.6 mg/kg) 腹腔注射给药给携带胆管癌肿瘤 EGI-1 的 SCID 小鼠,每天一次,持续两周。 结果:低剂量 Paclitaxel 治疗减少了 EGI-1 细胞的肺扩散,而不显著影响其局部肿瘤生长。[4] 方法:开发 Paclitaxel 醇诱导的负面情感症状的临床前模型,将 Paclitaxel (2-8 mg/kg in 1 volume ethanol+1 volume Emulphor-620 +18 volumes distilled water) 腹腔注射给 C57BL/6J 小鼠,每两天一次,共注射四次。 结果:8 mg/kg Paclitaxel 处理导致机械性和冷性异常性疼痛的发展和维持。Paclitaxel 也诱导了焦虑样行为和抑郁样行为。Paclitaxel 在小鼠情感状态建模试验中产生了行为改变,而伤害性反应的增加持续时间更长。[5] |
激酶实验 | To determine which caspases are involved in apoptosis induced by taxol, caspase-3 inhibitor (DEVD-CHO), caspase-6 inhibitor (Z-VEID-FMK), caspase-8 inhibitor (Z-IETD-FMK or IETD-CHO), caspase-9 inhibitors (Z-LEHD-FMK or LEHD-CHO), and caspase-10 inhibitor (Z-AEVD-FMK) are used. These caspase inhibitors are dissolved in dimethyl sulfoxide (Me2SO); the final concentration of Me2SO is 0.1%. Cells (5×105) are preincubated in the presence or absence of 100 μM?each of these inhibitors for 3 h at 37°C then treated with or without 0.1, 0.5, and 1 μM?Paclitaxel for 48 h and processed for annexin V binding assay [1]. |
细胞实验 | 1×10^4 cells are plated in 100 μL of the growth medium in the presence or absence of increasing concentrations (0.1-1 μM) of taxol in 96-well plates and cultured at 37°C in 5% CO2 for 12-48 h. The cells are then incubated with 25 μL of MTT (5 mg/mL) at 37°C for 4 h. After dissolving the crystals with 0.04 N HCl in isopropanol, the plates are read in a microplate reader at 570 nm [1]. |
动物实验 | Adult (250-320 g) male Sprague-Dawley rats are used for all experiments. One week following the DiI injection, rats are anesthetized with isofluorane and injected into the tail vein with 2 mg/kg paclitaxel or its vehicle (1:1:23, cremophor EL:ethanol:0.9% saline). The tail vein injection is repeated three more times every other day for a total of four injections [4]. |
别名 | 紫杉醇, Taxol, NSC 125973 |
分子量 | 853.91 |
分子式 | C47H51NO14 |
CAS No. | 33069-62-4 |
Smiles | CC(=O)O[C@@H]1C2=C(C)[C@H](C[C@@](O)([C@@H](OC(=O)c3ccccc3)[C@@H]3[C@@]4(CO[C@@H]4C[C@H](O)[C@@]3(C)C1=O)OC(C)=O)C2(C)C)OC(=O)[C@H](O)[C@@H](NC(=O)c1ccccc1)c1ccccc1 |
密度 | 1.39g/cm3 |
存储 | keep away from direct sunlight,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. | ||||||||||||||||||||||||||||||
溶解度信息 | Ethanol: 21.4 mg/mL (25 mM) 10% DMSO+40% PEG300+5% Tween 80+45% Saline: 8.54 mg/mL (10 mM), Suspension. Please add co-solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. DMSO: 41.66 mg/mL (48.79 mM), Heating is recommended. | ||||||||||||||||||||||||||||||
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中药材名称 | 中药材拉丁名 | 性 | 味 | 归经 |
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短叶红豆杉 | Taxus brevifolia | |||
海南粗榧 | Cephalotaxus hainanensis Li【C.mannii Hook.f.】 | 温 | 苦, 涩 | |
红豆杉 | Taxus chinensis (Pilg.)Rehd. var. mairei (Lemee et Levl.)Cheng et L. K. Fu(T. speciosa Florin) | |||
酱果紫杉 | Taxus baccata Linn. | |||
曼地亚红豆杉 | Taxus × media | |||
美丽红豆杉 | Taxus mairei (Lemée et Lévl.) S.Y.Hu | |||
喜马拉雅红豆杉 | Taxus wallichiana Zucc. | |||
云南红豆杉 | Taxus yunnanensis Cheng et L. K. Fu | |||
紫杉 | Taxus cuspidata Sieb. et Zucc. | 肾 |
中成药名称 | 处方组成 | 中成药类型 |
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方剂名称 | 处方组成 | 剂型 | 处方来源 |
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