SC75741 is a potent P65 inhibitor with IC50 of 200 nM

NF-κB:200 nM(EC50)

SC75741通过抑制人类PBMC增殖，展现出免疫抑制活性，IC50为2.2 μM。[1] 同时，SC75741通过在转录水平上抑制NF-κB介导的信号传导，抑制甲型和乙型流感病毒的复制。此外，SC75741在抵抗病毒变种发展方面表现出高阻力。[2]

SC75741（15 mg/kg i.p.）在小鼠感染H5N1流感病毒后，能有效减少小鼠肺部的病毒复制和细胞因子表达。[2]

NF-κB reporter gene assay: The NF-κB reporter gene assay is prepared with A549-NF-κB-SEAP cell line according to manufacturer's instructions. In short, A549 cells stably transfected with pNF-κB-SEAP reporter gene plasmid are plated and allowed to attach overnight. The cells are subsequently incubated for 5 h with described compounds at 100, 30, 10, 3, 1, 0.3, 0.1, and 0 μM and then stimulated with 10 ng/ml TNF-α for 22 h. The supernatant of the cell is analyzed for SEAP activity using a chemiluminescent SEAP reporter gene assay.

Cell viability assay is prepared using a CellTiter-BluTM Cell Viability Assay. For each concentration of the compound four replicates are measured.(Only for Reference)