ML324(IC50=920 nM) is a specific inhibitor of jumonji histone demethylase (JMJD2).

JMJD2:920 nM

ML324在Caco-2细胞中显示出良好的渗透能力，并在存在鼠和大鼠肝微粒体的情况下，具有优异的微粒体稳定性。ML324通过抑制病毒IE基因的表达，对抗单纯疱疹病毒（HSV）和人类巨细胞病毒（hCMV）感染展现出强大的抗病毒活性。[1]

在小鼠神经节外植模型中，用于研究潜伏感染小鼠，ML324抑制HSV斑块的形成，阻断HSV-1的重新激活。[1]

The fluorescence resonance energy transfer (FRET)-based LANCE Ultra KinaSelect Ser/Thr kit is used to determine IC50 values for various CDK inhibitors. Briefly, a specific ULight MBP peptide substrate (50 nM final concentration) is phosphorylated by a CDK-cyclin pair in buffer (50 mM HEPES-KOH pH 7.5, 10 mM MgCl2, 1 mM EGTA, 2 mM dithiothreitol) containing ATP at the concentration of the Km values of the individual kinases for 1 h at room temperature. Subsequently, phosphorylation is detected by addition of specific Eu-labelled anti-phospho-antibodies (2 nM), which upon binding to the phosphopeptide give rise to a FRET signal. FRET signals are then recorded[1].