购物车
  • 全部删除
  • TargetMol
    您的购物车当前为空

Danoprevir

Rating icon 很棒
产品编号 T6025Cas号 850876-88-9
别名 丹诺普韦, RO5190591, RG7227, R7227, ITMN-191

Danoprevir (RG7227) 是具有口服活性的NS3/4A 蛋白酶抑制剂,IC50值为 0.29 nM。它抑制 HCV 基因型 1a, 1b, 4, 5, 6 (IC50s=0.2-0.4 nM) 和 2b, 3a (IC50s=1.6, 3.5 nM)。它也是SARS-CoV 3CLpro 的抑制剂,IC50为 0.05 μM。

Danoprevir

Danoprevir

Rating icon 很棒
纯度: 99.52%
产品编号 T6025 别名 丹诺普韦, RO5190591, RG7227, R7227, ITMN-191Cas号 850876-88-9

Danoprevir (RG7227) 是具有口服活性的NS3/4A 蛋白酶抑制剂,IC50值为 0.29 nM。它抑制 HCV 基因型 1a, 1b, 4, 5, 6 (IC50s=0.2-0.4 nM) 和 2b, 3a (IC50s=1.6, 3.5 nM)。它也是SARS-CoV 3CLpro 的抑制剂,IC50为 0.05 μM。

规格价格库存数量
1 mg
¥ 410
现货
5 mg
¥ 997
现货
10 mg
¥ 1,580
现货
25 mg
¥ 2,710
现货
50 mg
¥ 3,950
现货
100 mg
¥ 5,750
现货
1 mL x 10 mM (in DMSO)
¥ 1,510
现货
大包装 & 定制
加入购物车
TargetMol 的所有产品仅用作科学研究或药证申报,不能被用于人体,我们不向个人提供产品和服务。请您遵守承诺用途,不得违反法律法规规定用于任何其他用途。
实验操作小课堂
常见问题解答
查看更多

"Danoprevir"的相关化合物库

选择批次:
纯度:99.52%
联系我们获取更多批次信息

产品介绍

生物活性
产品描述
Danoprevir (RG7227) is a peptidomimetic inhibitor of the NS3/4A protease(IC50=0.2-3.5 nM) of hepatitis C virus (HCV).
靶点活性
HCV NS3/4A protease:0.2 nM-3.5 nM
体内活性
在大鼠或猴子中,给予Danoprevir(30 mg/kg)。
激酶实验
Continuous fluorescent resonance energy transfer (FRET) assay: The assay buffer contains 25 μM NS4A peptide, 50 mM Tris-HCl, pH 7.5, 15% (vol/vol) glycerol, 0.6 mM lauryldimethylamine N-oxide, 10 mM dithiothreitol, and 0.5 μM fluorescein/QXL520-labeled FRET substrate {Ac-DE-Dap(QXL520)-EE-Abu-ψ-[COO]-AS-Cys(5-FAMsp)-NH2}. K2040 enzyme (50 pM) is added to initiate the reaction. Reactions are set up in black 96-well plates, and fluorescence data is collected. Control reactions lacking inhibitors and enzyme are included. Initial rates are calculated from the linear phase of the reaction (up to 1 hour) and are used to obtain IC50. Recovery of activity from preformed Danoprevir-NS3/4A complex is assessed by preincubating 10 nM NS3/4A with a two-fold excess of Danoprevir in 1× assay buffer for 15 min, followed by a rapid 200-fold dilution of the preformed complex into assay buffer containing substrate. A control reaction with the same final conditions without preincubation of NS3/4A and Danoprevir is initiated by the addition of enzyme to an otherwise-complete reaction mixture. Additional control reactions lack either Danoprevir or NS3. The progress of the reactions is followed over 5 hours.
细胞实验
Serially diluted Danoprevir is added to Huh7 cells harboring the K2040 replicon 1 day after cell plating. For antiviral assays, after a 48-hour incubation, intracellular RNA is extracted, and the level of HCV replicon RNA is quantified by reverse transcription (RT)-PCR assay with the primers (5'-CACTCCCCTGTGAGGAACTACTG-3' and 5'-AGGCTGCACGACACTCATACT-3') and a probe (5'-6-FAM-CTTCACGCAGAAAGCGTCTAGCCATGG-MGBNFQ-3' using an ABI Prism 7900 sequence detection system. Here, FAM is 6-carboxyfluorescin and MGBNFQ is a molecular-groove binding non-fluorescence quencher specific to the HCV 5' untranslated region. Single-tube reactions are performed using the TaqMan Gold RT-PCR kit. Triplicate reactions for the RNA standards and samples are performed in 50 μL with 5 μL intracellular RNA (50 ng). RT is carried out at 48 °C for 30 min followed by 10 min at 95 °C. The PCR is run as follows: 15 seconds at 95 °C and 1 min at 60 °C for 40 cycles. Each RNA concentration is determined in triplicate. The absolute concentration of replicon RNA is calculated based on its signal relative to that of a standard curve generated by known concentrations of an in vitro-transcribed RNA corresponding to a genotype 1b 5' untranslated region. Replicon levels in the presence of Danoprevir are fitted to a four-parameter logistic function to obtain EC50.(Only for Reference)
别名丹诺普韦, RO5190591, RG7227, R7227, ITMN-191
化学信息
分子量731.83
分子式C35H46FN5O9S
CAS No.850876-88-9
SmilesCC(C)(C)OC(=O)N[C@H]1CCCCC\C=C/[C@H]2C[C@]2(NC(=O)[C@@H]2C[C@H](CN2C1=O)OC(=O)N1Cc2cccc(F)c2C1)C(=O)NS(=O)(=O)C1CC1
密度1.41
储存&溶解度
存储Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
溶解度信息
H2O: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 133 mg/mL (181.74 mM), Sonication is recommended.
Ethanol: 133 mg/mL (181.74 mM), Sonication is recommended.
溶液配制表
DMSO/Ethanol
1mg5mg10mg50mg
1 mM1.3664 mL6.8322 mL13.6644 mL68.3219 mL
5 mM0.2733 mL1.3664 mL2.7329 mL13.6644 mL
10 mM0.1366 mL0.6832 mL1.3664 mL6.8322 mL
20 mM0.0683 mL0.3416 mL0.6832 mL3.4161 mL
50 mM0.0273 mL0.1366 mL0.2733 mL1.3664 mL
100 mM0.0137 mL0.0683 mL0.1366 mL0.6832 mL

SCI 文献

计算器

  • 摩尔浓度 计算器
  • 稀释 计算器
  • 配液 计算器
  • 分子量 计算器

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法:
TargetMol | Animal experiments比如您的给药剂量是 10 mg/kg ,每只动物体重 20 g ,给药体积 100 μLTargetMol | Animal experiments 一共给药动物 10 只 ,您使用的配方为 5% TargetMol | reagent DMSO+ 30%PEG300+ 5%Tween 80 + 60% ddH2O. 那么您的工作液浓度为 2 mg/mL
母液配置方法: 2 mg 药物溶于 50 μLDMSOTargetMol | reagent ( 母液浓度为 40 mg/mL ), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:50μLDMSOTargetMol | reagent 母液,添加 300 μLPEG300TargetMol | reagent 混匀澄清,再加 50μLTween 80, 混匀澄清,再加 600μLddH2OTargetMol | reagent 混匀澄清

以上为“体内实验配液计算器”的使用方法举例,并不是具体某个化合物的推荐配制方式,请根据您的实验动物和给药方式选择适当的溶解方案。

1 请输入动物实验的基本信息
mg/kg
g
μL
2 请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
% DMSO
%
%Tween 80
%ddH2O

剂量转换

对于不同动物的给药剂量换算,您也可以参考 更多

关键词

评论列表

4个月前
5.0
Rating icon 很棒

评论内容

Related Tags: buy Danoprevir | purchase Danoprevir | Danoprevir cost | order Danoprevir | Danoprevir chemical structure | Danoprevir in vivo | Danoprevir formula | Danoprevir molecular weight