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JNK-IN-7

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产品编号 T3598Cas号 1408064-71-0
别名 JNK inhibitor

JNK-IN-7 (JNK inhibitor) 是 JNK 抑制剂,抑制 JNK1、JNK2和 JNK3,IC50分别为 1.5、2 和 0.7 nM。

JNK-IN-7
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JNK-IN-7

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纯度: 99.58%
产品编号 T3598 别名 JNK inhibitorCas号 1408064-71-0

JNK-IN-7 (JNK inhibitor) 是 JNK 抑制剂,抑制 JNK1、JNK2和 JNK3,IC50分别为 1.5、2 和 0.7 nM。

规格价格库存数量
1 mg
¥ 865
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5 mg
¥ 2,180
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10 mg
¥ 2,830
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25 mg
¥ 4,660
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50 mg
¥ 6,490
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100 mg
¥ 8,780
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200 mg
¥ 11,800
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1 mL x 10 mM (in DMSO)
¥ 2,380
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产品介绍

生物活性
产品描述
JNK-IN-7 (JNK inhibitor) is a selective JNK1/2/3 inhibitor (IC50: 1.54/1.99/0.75 nM). It can also inhibit phosphorylation of c-Jun, which is a substrate of JNK kinase.
靶点活性
JNK3:0.75 nM, JNK1:1.54 nM, JNK2:1.99nM
体外活性
JNK-IN-7 is a relatively selective JNK inhibitor in cells. In addition to JNK 1, 2, 3, JNK-IN-7 also binds to IRAK1(IC50=14.1 nM), YSK4 (IC50=4.8 nM), ERK3 (IC50=22 nM), PIK3C3, PIP5K3 and PIP4K2C[1]. Expression of divalent metal-ion transporter 1 (DMT1) in HCT116 is demonstrated to be markedly decreased under stimulation with TNF for 24 and 48?h, while JNK-IN-7 can significantly reverse the decrease. TNF can down-regulate DMT1 expression, while JNK-IN-7 can markedly suppress this function[2].
激酶实验
A375 cells are pre-treated with 1 μM JNK-IN-7 for the indicated amounts of time. Remove the medium and wash 3 times with PBS. Resuspend the cell pellet with 1 mL Lysis Buffer (1% NP-40, 1% CHAPS, 25 mM Tris, 150 mM NaCl, Phosphatase Inhibitor Cocktail). Rotate end-to-end for 30 min at 4°C. Lysates are cleared by centrifugation at 14000 rpm for 15 min in the Eppendorf. The cleared lysates gel filtered into Kinase Buffer (0.1% NP-40, 20 mM HEPES, 150 mM NaCl, Phosphatase Inhibitor Cocktail, Protease Inhibitor Cocktail) using Bio-Rad 10DG colums. The total protein concentration of the gel-filtered lysate should be around 5-15 mg/mL. Cell lysate is labeled with the probe at 5 μM for 1 hour. Samples are reduced with DTT, and cysteines are blocked with iodoacetamide and gel filtered to remove excess reagents and exchange the buffer. Add 1 volume of 2X Binding Buffer (2% Triton-100, 1% NP-40, 2 mM EDTA, 2X PBS) and 50 μL streptavidin bead slurry and rotate end-to-end for 2 hours, centrifuge at 7000 rpm for 2 min. Wash 3 times with 1X Binding Buffer and 3 times with PBS. Add 30 μL 1X sample buffer to beads, heat samples at 95°C for 10 min. Run samples on an SDS-PAGE gel at 110V. After transferred, the membrane is immunoblotted with JNK antibody[1].
细胞实验
JNK-IN-7 is prepared in DMSO and stored, and then diluted with appropriate medium before use[2]. Intestinal epithelial cell line (HCT116) is cultured in DMEM medium, supplemented with 10% heat-inactivated fetal bovine serum (FBS), penicillin (100?U/mL) and streptomycin (100?g/mL), 2?mM L-gentamycin, and 50?μM 2-ME. These cells are stimulated with TNF (20?ng/mL), LPS (100?ng/mL), and IFN-γ (20?ng/mL), respectively. After 24 or 48?h of culture, cells are harvested followed by extraction of total RNA, and the levels of DMT1 mRNA are analyzed by qRT-PCR. To determine the mechanisms of TNF involved in regulating DMT1 expression, JNK-IN-7 (1?μM), NF-κB inhibitor (BAY 11-7082, 1?μM), and caspase-3/8 inhibitor (Z-DEVD-FMK, 50?μM) are also added into the culture medium. After 48?h of culture, cells are then collected to detect the expression of DMT1 by qRT-PCR[2].
别名JNK inhibitor
化学信息
分子量493.56
分子式C28H27N7O2
CAS No.1408064-71-0
SmilesCN(C)CC=CC(=O)Nc1cccc(c1)C(=O)Nc1ccc(Nc2nccc(n2)-c2cccnc2)cc1
密度1.301 g/cm3 (Predicted)
储存&溶解度
存储Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
溶解度信息
DMSO: 55 mg/mL (111.44 mM), Sonication is recommended.
溶液配制表
DMSO
1mg5mg10mg50mg
1 mM2.0261 mL10.1305 mL20.2610 mL101.3048 mL
5 mM0.4052 mL2.0261 mL4.0522 mL20.2610 mL
10 mM0.2026 mL1.0130 mL2.0261 mL10.1305 mL
20 mM0.1013 mL0.5065 mL1.0130 mL5.0652 mL
50 mM0.0405 mL0.2026 mL0.4052 mL2.0261 mL
100 mM0.0203 mL0.1013 mL0.2026 mL1.0130 mL

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TargetMol | Animal experiments比如您的给药剂量是 10 mg/kg ,每只动物体重 20 g ,给药体积 100 μLTargetMol | Animal experiments 一共给药动物 10 只 ,您使用的配方为 5% TargetMol | reagent DMSO+ 30%PEG300+ 5%Tween 80 + 60% ddH2O. 那么您的工作液浓度为 2 mg/mL
母液配置方法: 2 mg 药物溶于 50 μLDMSOTargetMol | reagent ( 母液浓度为 40 mg/mL ), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:50μLDMSOTargetMol | reagent 母液,添加 300 μLPEG300TargetMol | reagent 混匀澄清,再加 50μLTween 80, 混匀澄清,再加 600μLddH2OTargetMol | reagent 混匀澄清

以上为“体内实验配液计算器”的使用方法举例,并不是具体某个化合物的推荐配制方式,请根据您的实验动物和给药方式选择适当的溶解方案。

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