SW033291 is a small-molecule inhibitor of 15-PGDH (Ki=0.1 nM) that increases prostaglandin PGE2 levels in bone marrow and other tissues.

15-PGDH:0.1 nM(Ki), 15-PGDH:1.5 nM

在Vaco-503细胞中，SW033291 (2.5 μM) 通过降低85%的细胞内15-PGDH酶活性。[1]

在接受骨髓移植的小鼠中，SW033291（10 mg/kg，腹腔注射）促进了血液生成的恢复。在结肠和肝脏损伤的小鼠模型中，SW033291（10 mg/kg，腹腔注射）降低了与结肠炎相关的炎症细胞因子的水平，保护小鼠不受结肠炎影响，并促进了肝脏再生。[1]

Fluorescence anisotropy (FP) ligand displacement assay: All components are dissolved in buffer of composition 50 mM HEPES pH 7.4, 150 mM NaCl and 0.5 mM CHAPS with final concentrations of BRD 2/3/4 75 nM, fluorescent ligand 5 nM. 10 μL of this reaction mixture is added using a micro multidrop to wells containing 100 nL of various concentrations of I-BET151 or DMSO vehicle (1% final) in Greiner 384 well Black low volume microtitre plate and equilibrated in the dark for 60 minutes at room temperature. Fluorescence anisotropy is read in Envision (lex = 485 nm, lEM = 530 nm; Dichroic = 505 nM).