PHTPP is a selective ERβ antagonist.

PHTPP是一种选择性ERβ拮抗剂。PHTPP能够使FSH介导的cAMP产量降低80%（p<0.01），但对基础cAMP无影响。在10-6 M浓度下，PHTPP能够抑制E2刺激的ERβ活性，然而，它不抑制E2刺激的ERα活性。高剂量的PHTPP（10-6 M）会轻微增加1型Igf1 mRNA表达，并促进DPN诱导的1型Igf1 mRNA表达增加。

Cells are plated at a density of 3 to 4×10^5 in 1 mL of medium per well in a 24-well plate for cell viability and cAMP analysis. Cells are cultured in Dulbecco's Modified Eagle Medium with PHTPP (1 μM), or ethanol (0.1%) as the vehicle. The incubator is set to an atmosphere of 5% CO2 in air at 37°C, and cultures are allowed to acclimate for at least 24 h. The culture medium is then aspirated and replaced with serum-free DMEM/F12 containing 0.1 μM androstenedione. The cells are collected for intracellular cAMP and to test cell viability. They are for reference only.