Clitocine, an adenosine analogue derived from mushrooms, is a potent transcriptional readout agent, an inhibitor of nonsense mutations, and has anticancer activity, inducing the production of p53 protein in cells carrying the p53 nonsense mutation allele.Clitocine enhances TRAIL-mediated apoptosis in human colon cancer cells by promoting Mcl-1 degradation.NSC 81464 is an alkaloid of the yohimbine type extracted from the roots and leaves of Rauwolfia vomitoria.

Clitocine的加入对于早期终止密码子的读穿活性至关重要，并且在早期终止密码子的第三位置存在Clitocine足以促进强大的readthrough机制[2]。
Clitocine(0-0.8 μM；24小时)增强了LS411N和SW620细胞中TRAIL的致死性。 Clitocine(0.2μM；36小时)显著增强了TRAIL介导的凋亡[1]。

由Citocine (0.3-3 mg/kg; 皮下注射; 每周五次)诱导，p53在移植模型中抑制CAOV-33p53-UAA136肿瘤的生长[2]。