Vacquinol-1 is an MKK4 activator, which rapidly and selectively induces glioma cell death.

Vacquinol-1通过诱导膜褶皱、细胞圆化、大量巨大吞噬性空泡累积、ATP耗竭和细胞质膜破裂等机制，特异性快速引发胶质瘤细胞死亡，其IC50值为3.14 μM。[1]

在携带U3013 mg肿瘤的小鼠中，Vacquinol-1（15 μM颅内注射，或20 mg/kg经口给药）减缓了肿瘤生长并延长了存活时间。[1]

1MT enantiomers are solubilized in DMSO containing 0.1N HCl and added at concentrations of 100, 50, and 0 μM to wells containing the reaction mixture with tryptophan (0-200 μM) followed by addition of IDO enzyme. Plates are sealed and incubated 1 hr in a humidified 37°C incubator, after which the reactions are terminated by addition of 12.5 μl 30% TCA per well. Plates are then resealed in plastic wrap, incubated 30 min at 50°C to hydrolyze the reaction product N-formylkynurenine to kynurenine, and centrifuged. Supernatants are transferred to a flat-bottom 96-well plate, mixed with 100 μl Ehrlich reagent and incubated 10 min at room temperature. Absorbance at 490 nm is read[1].

Cell viability is measured using CellTiter-Glo Luminescent Cell Viability Assay according to the manufacturer's instructions.(Only for Reference)