Furazolidone (Furoxone), a nitrofuran derivative, inhibits AML1-ETO transformed cells with IC50 value of 12.7 μM. It is antibacterial and antiprotozoal activity,

AML1-ETO:12.7 μM

Furazolidone在亚微摩尔浓度下展现出强大的抗增殖特性，并在AML细胞系中诱导凋亡。对特定AML细胞的Furazolidone处理通过形态学和流式细胞术检测CD11b表达来诱导髓系细胞分化诱导 AML细胞中肿瘤抑制蛋白p53稳定性增加[1]。

FZ通过诱导CPR的活性，在鸡中加速自身的代谢，而在大鼠体内未观察到这种效果[2]。

Leukemic cells are seeded in 96-well culture plates at a density of 1 or 2×104 viable cells/100 µl/well in triplicates and are treated for 24, 48, and 72 hours with an incremental concentration of FZD ranging from 1 µM to 50 µM. Colorimetric CellTiter 96® Aqueous One Solution Cell Proliferation assay is used to determine the cytotoxicity. The optical density at 492 nm is measured using a Multiskan Ascent® microplate photometer. IC50 values are determined by MTS assay when cells are treated with FZD for 72 hours and calculated with GraphPad Prism 5. Each experiment was in triplicate. (Only for Reference)