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FIN56 是一种铁死亡特异性诱导剂,能结合并激活角鲨烯合酶,可通过诱导 GPX4 降解来诱导铁死亡。
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FIN56 是一种铁死亡特异性诱导剂,能结合并激活角鲨烯合酶,可通过诱导 GPX4 降解来诱导铁死亡。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
1 mg | ¥ 237 | 现货 | |
2 mg | ¥ 336 | 现货 | |
5 mg | ¥ 527 | 现货 | |
10 mg | ¥ 898 | 现货 | |
25 mg | ¥ 1,680 | 现货 | |
50 mg | ¥ 2,890 | 现货 | |
100 mg | ¥ 5,190 | 现货 | |
500 mg | ¥ 10,600 | 现货 | |
1 mL x 10 mM (in DMSO) | ¥ 623 | 现货 |
产品描述 | FIN56 is a specific inducer of ferroptosis. |
体外活性 | FIN56通过调节GPX4蛋白的丰度来触发铁死亡。它导致细胞裂解物中GPX4活性的丧失。通过过表达GFP-GPX4融合蛋白,可以抑制FIN56引起的细胞死亡。 |
细胞实验 | 1000 cells/36 μL are seeded in each well in 384-well plates. Lethal compounds are dissolved and a 2-fold, 12-point dilution series are prepared in DMSO. Compound solutions are further diluted with media at 1:25 and 4 μL/well of the diluted solutions are added to cell cultures immediately after cells are seeded. When ferroptosis inhibitors (100 μM α-tocopherol, 152 μM deferoxamine, or 10 μM U-0126) are co-treated with lethal inducers, they are supplemented to cell culture at the same time as lethal compounds are added, and the cells are incubated for 24 hrs. When other cell death modulating compounds (100 nM sodium selenite, 1 μM cerivastatin, 100 μg/mL mevalonic acid) are co-treated, they are first supplemented to cell culture for 24 hrs before lethal compounds are added to cell culture and further incubated for 24 hrs at 37°C under 5% CO2. On the day of the viability measurement, 10 μL/well of 50% Alamar Blue diluted in media is added and further incubated at 37°C for 6 hrs. Fluorescence intensity (ex/em: 530/590) is measured with a Victor 3 plate reader and the normalized viability is calculated by VL = (IL-I0)/(IV-I0), where VL, I0, IV, and IL are the normalized viability, raw fluorescence intensities from the wells containing media, cells treated with a vehicle (negative control), and cells with the lethal compound (L), respectively. When the effect of a chemical modulator (M) on L is calculated, we instead used the equation: VL|M = (IM, L-I0)/(IM, V-I0), where VL|M, IM, L and IM, V are the normalized viability, and fluorescence intensity from cells treated with M and V, and from cells with M and L. respectively. The viability is typically measured in biological triplicates unless otherwise specified. A representative dose-response curve, the mean and standard error of normalized viability from one replicate are plotted. |
分子量 | 517.66 |
分子式 | C25H31N3O5S2 |
CAS No. | 1083162-61-1 |
Smiles | ON=C1c2cc(ccc2-c2ccc(cc12)S(=O)(=O)NC1CCCCC1)S(=O)(=O)NC1CCCCC1 |
密度 | 1.50 g/cm3 (Predicted) |
存储 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. | ||||||||||||||||||||||||||||||
溶解度信息 | DMSO: 50 mg/mL (96.59 mM) | ||||||||||||||||||||||||||||||
溶液配制表 | |||||||||||||||||||||||||||||||
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