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CeMMEC1 是一种 BRD4 抑制剂,对 TAF1 有很好的亲和力,IC50为0.9 μM。
CeMMEC1 是一种 BRD4 抑制剂,对 TAF1 有很好的亲和力,IC50为0.9 μM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
1 mg | ¥ 130 | 现货 | |
5 mg | ¥ 295 | 现货 | |
10 mg | ¥ 496 | 现货 | |
25 mg | ¥ 1,090 | 现货 | |
50 mg | ¥ 1,930 | 现货 | |
100 mg | ¥ 2,860 | 现货 | |
500 mg | 询价 | 现货 | |
1 mL x 10 mM (in DMSO) | ¥ 328 | 现货 |
产品描述 | CeMMEC1 is an inhibitor of BRD4, and also has a great affinity for TAF1(IC50=0.9 μM). |
靶点活性 | TAF1:0.9 μM |
体外活性 | CeMMEC1 对 CREBBP、EP300、BRD9 的溴结构域以及 TAF1 的第二个溴结构域展现了高亲和性活性。 |
激酶实验 | TAF1 binding assays are conducted using the EPIgeneous Binding Domain kit B. Binding is determined by the displacement of an acetylated biotin peptide from a GST-tagged TAF1 protein using HTRF with a Eu3+-conjugated GST antibody donor and streptavidin-conjugated acceptor. Compounds (CeMMEC1) are dispensed into assay plates, ProxiPlate-384 Plus using an Echo 525 Liquid Handler. Binding assays are conducted in a final volume of 20 μL with 5 nM TAF1-GST, 50 nM peptide (SGRGK (ac)GGK (ac)GLGK (ac)GGAK (ac)RHRK (biotin)-acid), 6.25 nM Streptavidin-XL665, 1:200 Anti-GST-Eu3+ cryptate and 0.1% DMSO. Assay reagents are dispensed into plates using a Multidrop combi and incubated at room temperature for 3 h. Fluorescence is measured using a PHERAstar microplate reader using the HTRF module with dual emission protocol (A = excitation of 320 nm, emmission of 665 nm, and B = excitation of 320 nm, emission of 620 nm). Raw data are processed to give an HTRF ratio (channel A/B × 10,000), which is used to generate IC50 curves |
细胞实验 | REDS3 cells were treated with CeMMEC1(10 μM) incubating for 24 hours. |
别名 | CeMMEC1 HCl |
分子量 | 336.34 |
分子式 | C19H16N2O4 |
CAS No. | 440662-09-9 |
存储 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. | ||||||||||||||||||||
溶解度信息 | DMSO: 5.63 mg/mL (16.72 mM), Sonication is recommended. | ||||||||||||||||||||
溶液配制表 | |||||||||||||||||||||
DMSO
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