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AG1024

产品编号 T2693Cas号 65678-07-1
别名 Tyrphostin AG 1024, Tyrphostin, AGS 200

AG1024 (Tyrphostin) 是一种可逆的,竞争性和选择性的胰岛素样生长因子-1 受体抑制剂,IC50为 7 μM。它抑制胰岛素受体的磷酸化,IC50值为 57 μM。它诱导细胞凋亡并具有抗癌活性。

AG1024

AG1024

纯度: 99.37%
产品编号 T2693 别名 Tyrphostin AG 1024, Tyrphostin, AGS 200Cas号 65678-07-1

AG1024 (Tyrphostin) 是一种可逆的,竞争性和选择性的胰岛素样生长因子-1 受体抑制剂,IC50为 7 μM。它抑制胰岛素受体的磷酸化,IC50值为 57 μM。它诱导细胞凋亡并具有抗癌活性。

规格价格库存数量
1 mg¥ 218现货
5 mg¥ 496现货
10 mg¥ 745现货
25 mg¥ 1,250现货
50 mg¥ 1,860现货
100 mg¥ 2,980现货
1 mL x 10 mM (in DMSO)¥ 546现货
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纯度:99.37%
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产品介绍

生物活性
产品描述
AG1024 (Tyrphostin) suppresses IGF-1R autophosphorylation(IC50=7 μM), and is less potent for IR(IC50=57 μM).
靶点活性
Insulin receptor:57 μM, IGF-1R:7 μM
体外活性
在携带有Ba / F3-p210异种移植物小鼠中,AG-1024(30 μg)施用10天能够显著抑制肿瘤生长.
体内活性
AG-1024能够阻断IGF-1受体(IC50=7 μM)和IR(IC50=57 μM)自磷酸化。在MCF-7 细胞中,AG-1024 (10 μM) 能够能够下调磷酸化Akt1和bcl-2,上调Bax,p53和p21,从而抑制细胞增殖。
激酶实验
Inhibition of IGF-1- and insulin-stimulated cellular proliferation: NIH-3T3 cells overexpressing IGF-1 or insulin receptors are plated on 96-well plates (2,000-5,000 cells/well) and maintained overnight in complete medium. Cells are then changed to DMEM containing 1% FBS in the presence of 10 nM IGF-1 or insulin and various concentrations of AG-1024 for 120 hours. Medium is replaced every 48 hours. At the indicated periods of time, the medium is aspirated from the wells and 100 μL MTT is added to each well. The cells are then incubated for 4 hours at 37 °C and lysed by addition of 100 μL isoamylic alcohol and shaking for 20 minutes. The plate is then read in the ELISA reader at 570 and 690 nm. The IC50 value is calculated at the 120-hour time point.
细胞实验
Cells are exposed to AG-1024 for 24, 48 or 72 hours. For the determination of proliferation, cells are harvested and counted with trypan blue dye exclusion. Apoptosis is evaluated by dual staining of MCF-7 with fluoresceine anti-digoxigenin and propidium iodide. Briefly, fixed cells are washed with PBS, suspended in TdT buffer with TdT enzyme and Dig-dUTP for 60 minutes, and suspended in FITC blocking solution with anti-Dig-Fluorescein for 30 minutes at room temperature and kept in a dark place. Cells are then rinsed in buffer and resuspended in propidium iodide/RNase A solution for 30 minutes then analyzed by flow cytometry. For the assessment of phospho-Akt1, Bax, p53, bcl-2 and p21, cells are lysed and analyzed by western blot.(Only for Reference)
别名Tyrphostin AG 1024, Tyrphostin, AGS 200
化学信息
分子量305.17
分子式C14H13BrN2O
CAS No.65678-07-1
储存&溶解度
存储Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
溶解度信息
DMSO: 57 mg/mL (186.8 mM)
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
H2O: < 1 mg/mL (insoluble or slightly soluble)
溶液配制表
DMSO
1mg5mg10mg50mg
1 mM3.2769 mL16.3843 mL32.7686 mL163.8431 mL
5 mM0.6554 mL3.2769 mL6.5537 mL32.7686 mL
10 mM0.3277 mL1.6384 mL3.2769 mL16.3843 mL
20 mM0.1638 mL0.8192 mL1.6384 mL8.1922 mL
50 mM0.0655 mL0.3277 mL0.6554 mL3.2769 mL
100 mM0.0328 mL0.1638 mL0.3277 mL1.6384 mL

计算器

  • 摩尔浓度 计算器
  • 稀释 计算器
  • 配液 计算器
  • 分子量 计算器

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法:
TargetMol | Animal experiments比如您的给药剂量是 10 mg/kg ,每只动物体重 20 g ,给药体积 100 μLTargetMol | Animal experiments 一共给药动物 10 只 ,您使用的配方为 5% TargetMol | reagent DMSO+ 30%PEG300+ 5%Tween 80 + 60% ddH2O. 那么您的工作液浓度为 2 mg/mL
母液配置方法: 2 mg 药物溶于 50 μLDMSOTargetMol | reagent ( 母液浓度为 40 mg/mL ), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:50μLDMSOTargetMol | reagent 母液,添加 300 μLPEG300TargetMol | reagent 混匀澄清,再加 50μLTween 80, 混匀澄清,再加 600μLddH2OTargetMol | reagent 混匀澄清

以上为“体内实验配液计算器”的使用方法举例,并不是具体某个化合物的推荐配制方式,请根据您的实验动物和给药方式选择适当的溶解方案。

1 请输入动物实验的基本信息
mg/kg
g
μL
2 请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
% DMSO
%
%Tween 80
%ddH2O

剂量转换

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