Forsythoside B binds to LPS and reduces the biological activity of serum LPS, and inhibits NF-κB activation. Forsythoside B inhibits the inflammatory response and has antioxidant properties. Potent neuroprotective effects with a favorable therapeutic time-window, reduce of cerebral ischemia and reperfusion injury degree, attenuating blood-brain barrier (BBB) breakdown; Rescued cardiac function from I/R injury. Forsythoside B has antisepsis effect, is mediated by decreasing local and systemic levels of a wide spectrum of inflammatory mediators.

Forsythoside B在脂多糖（LPS）刺激的RAW264.7细胞中，通过浓度依赖的方式降低了TNF-α、IL-6和高迁移率族蛋白B1（HMGB1）的水平，抑制了IκB激酶（IKK）通路，并调节了核因子（NF）-κB[1]。

经静脉注射Forsythoside B单独或加入Imipenem可降低血清中TNF-α、IL-6、HMGB1、表达在骨髓细胞上的触发受体（TREM-1）及内毒素的水平，同时上调IL-10的血清水平，减少肺、肝、小肠中的髓过氧化物酶（MPO）[1]。超过8 mg/kg剂量的Forsythoside B在脑缺血和再灌注的大鼠中显示出显著的神经保护作用。20 mg/kg的Forsythoside B即使在脑缺血和再灌注后1小时、3小时和5小时延迟给药，也展示了显著的神经保护活性。20 mg/kg的Forsythoside B缩小了脑梗塞大小和脑水肿，降低了脑组织中Evans蓝的渗出量和髓过氧化物酶活性，抑制了脑中磷酸化IκB-α和NF-κB的表达[2]。Forsythoside B显著恢复心肌功能，提高LVSP和+/-dp/dt(max)。心肌梗死体积、血清中的Tn-T、TNF-α和IL-6水平、MDA含量和心肌组织中MPO活性都有所减少，蛋白HMGB1、磷酸化IκB-α和磷酸化NF-κB的表达下调，同时减轻了SOD和GPx活性的下降[3]。Forsythoside B单用或加Imipenem减少了大鼠CLP引起的死亡率。

Forsythoside B is dissolved in sterile saline solution and added to the medium at various concentrations (from 0.1 to 10 μM) and incubated with LPS stimulated RAW264.7 cells. Cell-free supernatants are collected after Forsythoside B treatment for 24 h. Cell viability is assessed by measuring lactate dehydrogenase (LDH) in the medium[1].