Afuresertib hydrochloride is an inhibitor of Akt kinase (Kis of 0.08/2/2.6 nM for Akt1/Akt2/Akt3 respectively)

Akt3:2.6 nM(Ki), Akt2:2 nM(Ki), Akt1:0.08 nM(Ki)

Afuresertib诱导的p21表达通过促进FOXO活性从而促使G1期阻滞。此外，afuresertib显著增强了顺铂诱导的细胞毒性。Afuresertib调节了与成纤维细胞核心血清反应相关的E2F1和MYC的表达。这表明afuresertib是治疗MPM[1]患者的有效抗癌化合物。

Cell confluence proliferation assay was performed using Incucyte ZOOM Live‐Cell Imaging System.?Briefly, ACC‐MESO‐4 and MSTO‐211H cells were seeded in 12‐well plates (cell density, 1 × 104 cells/well) and were incubated for 24 h at 37°C.?Then, the cells were incubated with 0, 2, 5, or 10 μmol/L afuresertib.?During treatment, cell growth was monitored by recording phase images using the IncuCyte ZOOM Live‐Cell Imaging System (magnification, ×100)[1].