360A is a stabilizing G-Quadruplex ligand, and also inhibits telomerase activity for telomerase in TRAP-G4 assay(IC50 : 300 nM).

Telomerase:300 nM

向A549肺癌细胞系处理5 μM 360A，可以在11天的治疗内抑制细胞增殖。使用新合成的二聚体(360A)2A处理，产生了相当的抗增殖效果[1]。

Supplemented with 10% fetal calf serum (FCS) and 1% penicillin/streptomycin. A549 cells were maintained in humidified incubators at 37 °C in an atmosphere containing 5% CO2 and ambient oxygen of 20%. Cells were grown in 25 cm^2 flasks (150,000 cells/flask) and treated with 5 μM of 360A or (360A)2A every 3 or 4 days (i.e., at each reseeding) until cultures were terminated. Control cells were either non-treated or treated with 0.1% DMSO. At each reseeding the remaining cells were pelleted and snap frozen for further DNA extraction.Statistical analysis was performed using GraphPad Prism 5. A Chi-square test was used to compare TDEs frequencies[1].