SCR7 pyrazine

产品编号 T1724Cas号 14892-97-8

别名 SCR7|||SCR7 吡嗪

SCR7 pyrazine (SCR7) 是一种 DNA 连接酶 IV 抑制剂，以连接酶 IV 依赖的方式阻断非同源末端连接。它也是 CRISPR/Cas9的增强子，可提高 Cas9 介导的同源性定向修复的效率。它诱导细胞凋亡，具有抗癌活性。

SCR7 pyrazine

产品编号 T1724别名 SCR7, SCR7 吡嗪Cas号 14892-97-8

规格	价格	库存
1 mg	¥ 286	现货
2 mg	¥ 397	现货
5 mg	¥ 672	现货
10 mg	¥ 987	现货
25 mg	¥ 1,970	现货
50 mg	¥ 2,930	现货
100 mg	¥ 4,330	现货
1 mL x 10 mM (in DMSO)	¥ 776	现货

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产品介绍

生物活性

产品描述	SCR7 pyrazine (SCR7) enhances CRISPR-Cas9-mediated homology-directed repair (HDR) efficiency in vitro up to 19-fold. Inhibits nonhomologous end-joining (NHEJ).
体外活性	SCR7在200μM及以上浓度下有效抑制多聚体的形成。SCR7成功抑制了MCF7、A549、HeLa、T47D、A2780、HT1080和Nalm6细胞的增殖，其IC50分别为40、34、44、8.5、120、10和50μM。[1] SCR7抑制了CRISPR-Cas9诱导的DSBs的NHEJ修复。[2]
体内活性	SCR7处理（10 mg/kg，肌肉注射）显著减少了乳腺腺癌诱导的肿瘤，并且与对照组相比，生命期增加了4倍。然而，在携带Dalton's淋巴瘤肿瘤模型的瑞士白鼠中，SCR7（20 mg/kg，腹腔注射）既不表现出肿瘤退缩也不增加生命期。在BALB/c小鼠中，SCR7（20 mg/kg，腹腔注射）显著增强了辐射、依托泊苷和3-氨基苯甲酰胺对源自Dalton's淋巴瘤（DLA）细胞的肿瘤的细胞毒作用。[1]
激酶实验	Complementation of SCR7 Inhibition with Puri?ed Ligase IV: Complementation experiment is carried out by adding increasing concentrations of puri?ed Ligase IV/XRCC4 complex (30, 60, and 120 fmol) along with the oligomeric DNA substrates (5' compatible and 5'-5' noncompatible ends) to the SCR7-treatedextracts. Reactions are incubated for 2 h at 25℃. The reaction products are then resolved on 8% denaturing PAGE. The gel is dried and exposed and the signal is detected with a PhosphorImager and analyzed with Multi Gauge (V3.0) software.
细胞实验	Cell proliferation of cancer cells are determined by MTT and trypan blue assays. Briefly, MCF7, CEM, HeLa, A549, HT1080, A2780, T47D, Nalm6, N114 and K562 cells are grown in presence of SCR7 (10, 50, 100, and 250?μM) for 24 or 48?h, and subjected to MTT or trypan blue assays. Each experiment is repeated a minimum of three independent times. (Only for Reference)
别名	SCR7, SCR7 吡嗪

偶联与修饰

抗原信息

存储 & 运输

化学信息

分子量	332.38
分子式	C₁₈H₁₂N₄OS
CAS No.	14892-97-8

储存&溶解度

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

溶解度信息

H2O: < 1 mg/mL (insoluble or slightly soluble)

Ethanol: 3 mg/mL (9.03 mM)

DMSO: 66 mg/mL (198.57 mM)

溶液配制表

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	3.0086 mL	15.0430 mL	30.0860 mL	150.4302 mL
5 mM	0.6017 mL	3.0086 mL	6.0172 mL	30.0860 mL

DMSO

	1mg	5mg	10mg	50mg
10 mM	0.3009 mL	1.5043 mL	3.0086 mL	15.0430 mL
20 mM	0.1504 mL	0.7522 mL	1.5043 mL	7.5215 mL
50 mM	0.0602 mL	0.3009 mL	0.6017 mL	3.0086 mL
100 mM	0.0301 mL	0.1504 mL	0.3009 mL	1.5043 mL