Seco-DUBA, a duocarmycin (DUBA) prodrug, possess two hydroxyl groups suitable for antibody coupling through a linker. This compound, serving as a precursor, enables the synthesis of antibody-drug conjugates (ADCs).

Seco-DUBA (SK-BR-3 cells; 0.0001 pM~0.01 nM; 144 hours) dose-dependent reduces cell viability and shows equally potent and efficacious as DUBA[1]. Seco-DUBA causes SK-BR-3 (IC 50 =0.09), SK-OV-3 (IC 50 =0.43), and SW620 (IC 50 =0.09) cells to exhibit highly sensitive[1]. Cell Cytotoxicity Assay[1]Cell Line: SK-BR-3 cells Concentration: 0.0001 pM~0.01 nM Incubation Time: 144 hours Result: Dose-dependent reduced cell viability.

Seco-DUBA (89 μg/kg; i.v.) is likely converted to DUBA almost instantaneously[1]. Animal Model: Wistar rats[1]Dosage: 89 μg/kg (Pharmacokinetic Analysis) Administration: I.v. Result: Likely converted to DUBA almost instantaneously.