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c-Kit-IN-1

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产品编号 T4332Cas号 1225278-16-9
别名 PDGFR inhibitor 1, DCC-2618

c-Kit-IN-1 (DCC-2618) 是c-Kit 和c-Met 抑制剂(IC50<200 nM)。

c-Kit-IN-1

c-Kit-IN-1

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纯度: 98.72%
产品编号 T4332 别名 PDGFR inhibitor 1, DCC-2618Cas号 1225278-16-9

c-Kit-IN-1 (DCC-2618) 是c-Kit 和c-Met 抑制剂(IC50<200 nM)。

规格价格库存数量
1 mg¥ 378现货
2 mg¥ 548现货
5 mg¥ 898现货
10 mg¥ 1,490现货
25 mg¥ 3,230现货
50 mg¥ 4,770现货
100 mg¥ 6,780现货
500 mg¥ 13,600期货
1 mL x 10 mM (in DMSO)¥ 978现货
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产品介绍

生物活性
产品描述
c-Kit-IN-1 (DCC-2618) is an effective inhibitor of c-Met and c-Kit (IC50s<200 nM).
靶点活性
c-Kit:<200 nM, c-Met:<200 nM
体外活性
DCC-2618还能抑制KDR和PDGFR α/β(IC50s:<2 μM、<10 μM和<10 μM)。
激酶实验
The activity of c-KIT kinase is determined by following the production of ADP from the kinase reaction through coupling with the pyruvate kinase/lactate dehydrogenase system. In this assay, the oxidation of NADH (thus the decrease at A340 nM) is continuously monitored spectrophotometrically. The reaction mixture (100 μL) contained c-KIT (cKIT residues T544-V976, from ProQinase, 5.4 nM), polyE4Y (1 mg/mL), MgC12 (10 mM), pyruvate kinase (4 units), lactate dehydrogenase (0.7 units), phosphoenol pyruvate (1 mM), and NADH (0.28 mM) in 90 mM Tris buffer containing 0.2 % octyl-glucoside and 1% DMSO, pH 7.5. Test compounds (e.g., DCC-2618) are incubated with c-KIT and other reaction reagents at 22 °C for < 2 min before ATP (200 μM) is added to start the reaction. The absorption at 340 nm is monitored continuously for 0.5 hours at 30 °C on Polarstar Optima plate reader (BMG). The reaction rate is calculated using the 0 to 0.5 h time frame. Percent inhibition is obtained by comparison of reaction rate with that of a control (i.e. with no test compound).
细胞实验
DCC-2618 is prepared in DMSO and stored, and then diluted with the appropriate medium before use.A serial dilution of test compounds (e.g., DCC-2618) is dispensed into a 96-well black clear bottom plate. For each cell line, five thousand cells are added per well in 200 μL complete growth medium. Plates are incubated for 67 hours at 37 degrees Celsius, 5% CO2, 95% humidity. At the end of the incubation period, 40 μL of a 440 μM solution of resazurin in PBS is added to each well and incubated for an additional 5 hours at 37 degrees Celsius, 5% CO2, 95% humidity. Plates are read on a Synergy2 reader using an excitation of 540 nM and an emission of 600 nM.
别名PDGFR inhibitor 1, DCC-2618
化学信息
分子量489.47
分子式C26H21F2N5O3
CAS No.1225278-16-9
SmilesCn1cc(cn1)-c1cc(Oc2cc(F)c(NC(=O)C3(CC3)C(=O)Nc3ccccc3)cc2F)ccn1
密度1.40 g/cm3 at 20℃
储存&溶解度
存储Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
溶解度信息
DMSO: 88 mg/mL (179.8 mM)
溶液配制表
1mg5mg10mg50mg
1 mM2.0430 mL10.2151 mL20.4303 mL102.1513 mL
5 mM0.4086 mL2.0430 mL4.0861 mL20.4303 mL
10 mM0.2043 mL1.0215 mL2.0430 mL10.2151 mL
20 mM0.1022 mL0.5108 mL1.0215 mL5.1076 mL
50 mM0.0409 mL0.2043 mL0.4086 mL2.0430 mL
100 mM0.0204 mL0.1022 mL0.2043 mL1.0215 mL

计算器

  • 摩尔浓度 计算器
  • 稀释 计算器
  • 配液 计算器
  • 分子量 计算器

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法:
TargetMol | Animal experiments比如您的给药剂量是 10 mg/kg ,每只动物体重 20 g ,给药体积 100 μLTargetMol | Animal experiments 一共给药动物 10 只 ,您使用的配方为 5% TargetMol | reagent DMSO+ 30%PEG300+ 5%Tween 80 + 60% ddH2O. 那么您的工作液浓度为 2 mg/mL
母液配置方法: 2 mg 药物溶于 50 μLDMSOTargetMol | reagent ( 母液浓度为 40 mg/mL ), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:50μLDMSOTargetMol | reagent 母液,添加 300 μLPEG300TargetMol | reagent 混匀澄清,再加 50μLTween 80, 混匀澄清,再加 600μLddH2OTargetMol | reagent 混匀澄清

以上为“体内实验配液计算器”的使用方法举例,并不是具体某个化合物的推荐配制方式,请根据您的实验动物和给药方式选择适当的溶解方案。

1 请输入动物实验的基本信息
mg/kg
g
μL
2 请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
% DMSO
%
%Tween 80
%ddH2O

剂量转换

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