7,8-Dihydroxyflavone (7,8-DHF) is a naturally-occurring flavone and exist in Tridax procumbens, Godmania aesculifolia, and primula tree leaves.

TrkB receptor:320 nM(Kd)

7,8-DHF是一种特异性激活TrkB的正向化合物，不激活TrkA或TrkC，浓度为250 nM。除了皮质和海马神经元外，7,8-DHF还保护包括RGC（视网膜神经节细胞）和PC12细胞在内的其他细胞类型，使其免受兴奋毒性和氧化应激诱导的凋亡和细胞死亡。因此，它具有神经保护特性[1]。

7,8-Dihydroxyflavone是一种具有生物活性的化学物质，能够通过血脑屏障(BBB)影响小鼠大脑中TrkB及其下游的PI3K/Akt和MAPK激活，通过腹腔或口服给药方式实现。7,8-DHF在创伤性脑损伤的皮质神经元中促进存活，减少凋亡，7,8-DHF在伤后3小时给药可以通过PI3K/Akt途径减少脑组织损伤。其治疗未引起小鼠明显的毒性反应，在慢性治疗期间对小鼠无毒性。7,8-DHF在治疗阿尔茨海默病方面显示出强大的疗效，并通过激活肌肉中的TrkB[1]抑制肥胖。

PC12 cells are seeded in 96-well plates at 104/well. After pretreatment with 7,8-DHF (1-25 μM) for 1 h, the cells are exposed to 6-OHDA (100 μM) for subsequent 24 h. The PI3k inhibitor LY294002 or MEK inhibitor PD98059 is added 30 min before 7,8-DHF treatment. At the end of the experiment, PC12 cells are incubated with 20 μl of MTT solution (5 mg/ml in PBS) for 4 h at 37 ?C. The dark blue formazan product due to the reduction of MTT is dissolved in 150 μl of DMSO, and the absorbance at 570 nm is recorded with a microplate reader. The viability is expressed as the percentage of the untreated control cells. (Only for Reference)