Z-IETD-FMK (Z-IE(OMe)TD(OMe)-FMK) is a selective caspase 8 inhibitor, which has cell permeable.

Z-IETD-FMK通过抑制caspase-8的切割以及部分抑制caspase-3和PARP的切割，防止了暴露于不同凋亡刺激下的视网膜细胞的凋亡执行。[1] Z-IETD-FMK (50 μM) 降低神经酰胺诱导的心肌细胞死亡，并显著抑制caspase 3的激活。[2] Z-IETD-FMK对caspase-8的抑制作用影响了激活/记忆T细胞的生成和T细胞细胞因子的产生，并减少了T细胞培养中对TCR:CD3结合的NF-kappaB反应。[3]

在体内，Z-IETD-FMK通过抑制caspase-8能够减少记忆/活化的CD4与CD8 T细胞，并增加对T. cruzi感染的易感性。[3] Z-IETD-FMK促进中枢神经系统受伤后神经元的存活以及受损视网膜神经节细胞的再生。[4]

Proteasome assay: Exponentially growing cells on a 96-well clustered plate are treated with different concentrations of drugs or left untreated (control) for 6 hours. Proteasomes extracted with 0.5% NP40 buffer are mixed with equal amounts of samples in 100 μL total volume, and then incubated with 25 μmol/L of fluorogenic substrates (LRR- specific for trypsin-like activity, LLE-specific for caspase-like activity, and SUVY-specific for chymotrypsin-like activity) in black-bottom 96-well plates at 37°C. Fluorescence is monitored every 5 minutes at the wavelength of 360 nm (excitation) and 480 nm (emission).