Ac-DEVD-AMC

产品编号 T18909Cas号 169332-61-0

别名 AC-ASP-MET-GLN-ASP-AMC|||AC-ASP-MET-GLN-ASP-7-AMINO-4-METHYLCOUMARIN

Ac-DEVD-AMC (AC-ASP-MET-GLN-ASP-7-AMINO-4-METHYLCOUMARIN) 是一种 Caspase-3底物。

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产品编号 T18909别名 AC-ASP-MET-GLN-ASP-AMC, AC-ASP-MET-GLN-ASP-7-AMINO-4-METHYLCOUMARINCas号 169332-61-0

Ac-DEVD-AMC (AC-ASP-MET-GLN-ASP-7-AMINO-4-METHYLCOUMARIN) 是一种 Caspase-3底物。

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生物活性

产品描述	Ac-DEVD-AMC (AC-ASP-MET-GLN-ASP-7-AMINO-4-METHYLCOUMARIN) is the substrate of Caspase-3 .
体外活性	AMC基团在2小时时达到最高，面积为5.6±1.8。Ac-DEVD-AMC在不含肺部样本的情况下（仅底物）孵育6小时，峰值面积相对较小[1]。Ac-DEVD-AMC可用于监测细胞内caspase-3活性[2]。
细胞实验	Lung fragments (about 20 mg each) are collected from rats and mice. The samples are incubated at 37°C in 50 mL KH buffer or MEM (continuously gass with 95% O2: 5% CO2) for up to 6 h. At specific time points, samples are incubated in oxygenated KH buffer or MEM with 32 μM zVAD-fmk or 15 μL DMSO for 20 min (f/v=1.0 mL). Ac-DEVD-AMC (37 μM) is then added and the incubation continues for an additional 20 min. At the end of incubation, the tissue is disrupted by vigorous homogenization for 2 min, sonication for 3 min, and 10 passages through a 27-G needle. This disruption procedure quenches the Ac-DEVD-AMC cleavage reaction due to dilution. The supernatants are collected by centrifugation (~6,300g for 90 min) through a microcentrifuge filter, separated on HPLC, and analyzed for the fluorogenic AMC moiety [2].
别名	AC-ASP-MET-GLN-ASP-AMC, AC-ASP-MET-GLN-ASP-7-AMINO-4-METHYLCOUMARIN