TH34 is an HDAC6/8/10 inhibitor (IC50s: 4.6/1.9/7.7 μM). It shows high selectivity over HDAC1/2/3.

HDAC6:4.6 μM, HDAC8:1.9 μM, HDAC10:7.7 μM

TH34 在高达25 μM的浓度下，被非转化的人类皮肤成纤维细胞良好耐受，并在髓母细胞瘤细胞系中适度抑制集落生长，但特别是在几种人类神经母细胞瘤细胞系中，以浓度依赖的方式特异性诱导caspase依赖的程序性细胞死亡。

HeLa cells, stably transfected with NanoBRET plasmids NanoLuc?-HDAC6 FL Fusion Vector and NanoLuc?-HDAC10 FL Fusion Vector were seeded at 20,000 cells/well in white 96-well plates. Without further incubation, tracer (0.3 μM) and drugs were added in separate steps and plates were placed in a tissue culture incubator for 2 h. For NanoBRET quantification, plates were put at room temperature for 10 min. Nanoglow substrate, diluted in OptiMEM without phenol red, was added and measured within 10 min in an OPTIMA plate reader (460 nm emission for donor and 610LP filter for acceptor). The BRET signal was calculated by the ratio of acceptor signal to donor signal.