Nomilin is naturally occurring triterpenoids, have immunomodulatory activity.

Nomilin显著抑制内皮细胞的增殖、侵袭、迁移和管腔形成。它还对包括白血病（HL-60）、卵巢（SKOV-3）、肝脏（Hep G2）、宫颈（HeLa）、胃（NCI-SNU-1）和乳腺（MCF-7）在内的多种人类癌症细胞系具有抗增殖活性[2]。与对照组相比，Nomilin显著降低了TRAP阳性多核细胞的数量，并未表现出细胞毒性。它降低了骨吸收活性，并下调了特异性破骨细胞基因NFATc1和TRAP mRNA水平。此外，Nomilin抑制了MAPK信号传导途径。因此，Nomilin在体外对破骨细胞的分化具有抑制作用[3]。

Nomilin 在小鼠体内有效诱导谷胱甘肽S-转移酶活性，同时能够抑制仓鼠颊囊癌变。Nomilin 还能缩短小鼠因麻醉剂诱导的睡眠时间，这可能是通过对中枢神经系统的刺激作用实现的[1]。Nomilin 显著抑制了向肿瘤方向的毛细血管形成。经Nomilin 处理后，血清中的促炎细胞因子如IL-1β、IL-6、TNF-α以及GM-CSF和血清中的NO水平显著降低。Nomilin 的给药显著降低了血清中促血管生成因子VEGF的水平，并提高了抗血管生成因子TIMP-1和IL-2的水平[2]。

Cell lines: HUVECs. Concentrations: 5 μg-500 μg/ml. Incubation Time: 48 h. Method:. HUVECs were seeded (5000 cells/well) in 96-well flat-bottomed titer plate and incubated for 24 h at 37 °C in 5% CO2 atmosphere. Different concentrations of Nomilin (5 μg-500 μg/ml) were added and incubated further for 48 h. Before 4 h completion of incubation, 20 μl MTT (5 mg/ml) was added. Percentage of dead cells was determined using an ELISA plate reader set to record absorbance at 570 nm.

Animal Models: Four to six week old male C57BL/6 mice. Formulation: light paraffin oil. Dosages: 6 mg/kg, I.P.