SC79 is an AKT agonist with specificity and blood-brain barrier permeability. SC79 specifically binds to the PH domain of AKT, activates cytoplasmic AKT, and inhibits AKT membrane translocation. SC79 has neuroprotective activity.

方法：人宫颈癌细胞 HeLa 血清饥饿 1 h，用 SC79 (4 µg/mL) 处理 30 min，使用 Western Blot 检测靶点蛋白表达水平。
结果：SC79 增强了 AKT 磷酸化，SC79 诱导的 AKT 磷酸化主要发生在胞质中。[1]
方法：人肺癌细胞 A549 用 SC79 (10 µg/mL) 处理 24 h，使用 qPCR 检测基因表达水平。
结果：SC79 处理上调了 Nrf-2 (NFE2L2) 基因本身以及下游靶标 HO-1 和 NQO-1 的表达。[2]

方法：为检测体内活性，将 SC79 (0.04 mg/g) 腹腔注射给 C57 Black/6 小鼠铜松脱髓鞘模型，5 min 后进行大脑中动脉闭塞 (MCAO) 构建缺血性中风模型。
结果：单次剂量的 SC79 在 MCAO 后 24 h 和 MCAO 后 1 周将新皮质病变的大小分别减少 35% 和 40% 以上。[1]
方法：为研究对肝损伤的作用，将 SC79 (10 mg/kg) 腹腔注射给 C57BL/6 小鼠，0.5 h 后注射 d-Gal/LPS 诱导肝损伤。
结果：SC79 保护小鼠免受 d-Gal/LPS 诱导的 TNF-α 介导的肝损伤。[3]

Cytosolic phosphorylation of Akt: Hela cells are serum starved for 1 hr and treated with IGF (100ng/mL) or SC79 (4 μg/mL) for 30 minutes. Cells are lysed in Lysis buffer containing 250 mM Sucrose, 20 mM HEPES, 10 mM KCl, 1.5 mM MgCl2, 1 mM EDTA, 1 mM EGTA supplemented with protease inhibitors. Cells are passed through 25 g needle several times and kept on ice for 20 minutes. Total cell lysate is taken at this point. Cell lysates are centrifuged at 100,000 g for 30 minutes. Supernatant is collected as the cytosolic fraction. Pellet is washed with lysis buffer and represents the membrane fraction. Total cell lysate, cytosolic and membrane fractions are resolved by SDS-PAGE and analyzed for phospho-Akt (S473), Total Akt, Tubulin (cytosolic marker) and Orai1 (membrane marker) by western blotting.

HsSultan or NB4 cells (2.5 × 105) are plated in a 24-well plate in 500 μL of phenol red-free RPMI medium supplemented with 10% FBS. After incubation for 24 hours, each compound (8 μg/mL) is added and cultured for overnight (16–20 h). Fifty microliters of MTT solution (5 mg/mL in PBS) are added to each well. Following 2 hrs incubation, the purple formazan crystals are dissolved by directly adding in 500 μL of isopropanol with 0.1 M HCl to each well. After clearing the cell debris by centrifugation, the absorbance is measured at a wavelength of 570 nm.(Only for Reference)