Piperine (Bioperine) , a alkaloid, has been used in trials studying the treatment of multiple myeloma and deglutition disorders.

CYP3A4:61.94±0.054 μg/mL, P-gp:61.94±0.054 μg/mL

对于动物以不同的给药方式,Piperine的LD50不同.对于小鼠,静脉注射（LD50=15.1 mg/kg）,腹腔注射（LD50=43 mg/kg）,皮下注射（LD50=200 mg/kg）,灌胃给药（LD50=330 mg/kg）.对于大鼠,腹腔注射（LD50=33.5 mg/kg）,灌胃给药（LD50=514 mg/kg）

Magnolol具有抗真菌活性,对于须癣毛癣菌,石膏样小孢子菌,絮状表皮癣菌,黑曲霉菌,隐球菌,以及白色念珠菌均能起抑制作用（MIC=25-100μg/ml）。在体外,Magnolol增加(3)H-蝇蕈醇与海马体的结合。

Standard solution is prepared by dissolving 10 mg of piperine in 100 mL of methanol. The MTT assay is carried out to measure cell viability. Ten thousand cells in 100 μL of DMEM media are seeded in the wells of a 96-well plate. After 24 h, existing media is removed and 100 μL of various concentrations of piperine (20–100 μg/mL) are added and incubated for 48 h at 37 °C in a CO2 incubator. Control cells are supplemented with 0.05 % DMSO vehicle. At the 48th hour of incubation, MTT (10 μL of 5 mg/mL) is added to the plate. The contents of the plate are pipetted out carefully, the formazan crystals formed are dissolved in 100 μL of DMSO, and the absorbance is measured at 550 nm in a microplate reader[1].