L-quebrachitol is a natural product.L-quebrachitol may promote osteoblastogenesis by triggering the BMP-2-response as well as the Runx2, MAPK, and Wnt/β-catenin signaling pathway.

L-quebrachitol显著促进细胞增殖和DNA合成，同时增强由骨基质蛋白mRNA表达上调所伴随的矿化作用，包括碱性磷酸酶（ALP）、I型胶原（ColI）、骨钙素（OCN）和骨桥蛋白（OPN）。此外，L-quebrachitol上调骨形态发生蛋白-2（BMP-2）和runt相关转录因子-2（Runx2）的mRNA及蛋白表达，同时下调核因子-κB配体受体（RANKL）mRNA水平。还有，与丝裂原活化蛋白激酶（MAPK）和Wnt/β-catenin信号通路相关的调控基因表达也被上调。通过激活BMP-2响应以及Runx2、MAPK和Wnt/β-catenin信号通路，L-quebrachitol可能促进成骨细胞生成。

The total RNA from cells treated with l-quebrachitol was extracted using the TriPure Isolation Reagent .RNA (1 μg) was reverse transcribed using the Transcriptor First Strand cDNA Synthesis Kit to cDNA.The qRT-PCR analysis was performed using the EvaGreen HRM Mix .GAPDH was used as an internal control.The relative expression ratio was calculated using the 2^?△△Ct method.