CaCCinh-A01 is an inhibitor of the calcium-activated chloride channel (CaCC, 10 μM) and TMEM16A (IC50: 2.1 μM).

TMEM16A:2.1 μM, CaCC:10 μM

单宁酸（100 μM）和CaCCinh-A01（30 μM）有效抑制ATP刺激后的CaCC电流[1]。CaCCinh-A01以不同浓度（0.1/1/10 μM）分别降低钙依赖性氯流（38±14%、66±10%、91±1%）。在10 μM和30 μM CaCCinh-A01作用下，ATP诱导的短路电流分别降低38±7%和78±3%。

Each well of a 96-well plate is washed three times with PBS (200 μL/wash), leaving 50 μL of PBS. Test compounds (including CaCCinh-A01) (0.5 μL) are added to each well at 25 μM final concentration. After 10 min, 96-well plates are transferred to a plate reader for fluorescence assay. Each well is assayed individually for TMEM16A-mediated I- influx by recording fluorescence continuously (400 ms/point) for 2 s (baseline), then 50 μL of a 140 mM I-solution containing 200 μM ATP is added.