CB1954(Tretazicar (NSC-115829)), an anticancer prodrug, is activated by NAD(P)H quinone oxidoreductase 2. It is converted in the presence of the enzyme NQO2 and co-substrate caricotamide ( EP-0152R) (EP) into a potent cytotoxic bifunctional alkylating agent.

在NPC细胞系CNE1中，Tretazicar增强了细胞杀伤效果。在HepG2细胞系中，NOR1基因上调Grb2表达并激活MAPK信号转导，从而增强Tretazicar介导的细胞毒性。

NTR/CB1954系统通过剂量依赖的方式，用于体内特异性细胞的消融。NTR介导的CB1954活化产生交联，推测是通过触发凋亡级联反应迅速导致细胞死亡。NTR-CB1954的选择性和强效细胞杀伤作用不需要p53功能。

HepG2 cells,which are maintained in RPMI 1640 supplemented with 10% fetal calf serum (FCS) in a humidified culture incubator at 37?C with 5% CO2 and 95% air, grow to ~80% confluence are washed with PBS and treated with r CB1954(4-10 μmol/L) for 48hours.

RED 40 female mice,which express high levels of BLG-NTR transgene in the mammary gland and nontransgenic control mice on lactation day 6, were injected intraperitoneally （i.p.）with 50 mg/kg CB1954 dissolved in arachis oil containing 10% acetone.