C-DIM12 induced expression of Nurr1-regulated genes. C-DIM12 increased expression of transfected human Nurr1, induced Nurr1 protein expression in primary dopaminergic neurons and enhanced neuronal survival from exposure to 6-OHDA.

C-DIM12在细胞系和原代神经元中诱导Nurr1和DA基因表达[3]。此化合物还能抑制体外星形胶质细胞的炎症信号。C-DIM12抑制脂多糖(LPS)诱导的BV-2微胶质细胞中NF-κB调控基因的表达，包括一氧化氮合酶(NOS2)、白细胞介素-6(IL-6)及趋化因子(C-C动机)配体2(CCL2)，其效果通过Nurr1-RNA干扰而减弱。此外，C-DIM12降低了NF-κB-GFP（绿色荧光蛋白）报告细胞中NF-κB的激活，并增强了Nurr1在原代小胶质细胞核内的转移。C-DIM12减少了脂多糖诱导的p65与NOS2启动子的结合，并同时增强了Nurr1与p65结合位点的结合。C-DIM12还稳定了对抑制因子1沉默转录因子（CoREST）和核受体辅助抑制蛋白2（NCOR2）[2]的结合。

C-DIM12在MPTPp处理的小鼠中具有神经保护活性[2]。

NF-κB–GFP HEK cells are exposed to 30 ng/ml of TNFα in the presence of 100 μM C-DIM12 for up to 24 hours. C-DIM12 is efficient at blocking NF-κB–GFP expression in the NF-κB–GFP HEK cells after TNFα treatment, displaying a statistically significant reduction in total GFP fluorescence per cell.(Only for Reference)