JW 55 (JW55) is an effective and selective β-catenin signaling pathway inhibitor, works by inhibition of the PARP domain of tankyrase 1 and tankyrase 2 (TNKS1/2).

TNKS2 (auto-PARsylation):830 nM, TNKS1 (auto-PARsylation):1.9 μM

JW55是一种针对经典Wnt通路的强效且选择性的抑制剂。在Wnt3a诱导并瞬时转染有ST-Luc（SuperTop-萤火虫素酶）报告基因的HEK293细胞中，JW55展现出以IC50值为470 nM的抑制作用。在SW480细胞中，JW55在1至5μM范围内有效；而在HCT-15细胞中，有效范围扩展至0.01至5μM[1]。

JW 55（100 mg/kg，口服）减缓了条件性Apc基因敲除小鼠的肿瘤发展。JW 55减少了Xenopus胚胎中XWnt8诱导的轴向复制和Tamoxifen诱导的条件性APC突变小鼠中的息肉形成[1]。

JW 55 (JW55) is prepared in DMSO and stored, and then diluted with appropriate medium[1]. A total of 1,000 SW480 or RKO cells are seeded in 96-well plates. The day after, the cell culture medium is exchanged to solutions that contained 0.1% DMSO or 10 μM JW55 for RKO cells and 0.1% DMSO or 10, 5, or 1 μM JW55 for SW480 cells. All samples consist of a minimum of 6 replicates. The plate is incubated in an IncuCyte inside a cell culture incubator. Images are captured every second hour to monitor proliferation[1].