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Thiazovivin

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产品编号 T2155Cas号 1226056-71-8

Thiazovivin 是一种ROCK 抑制剂,能提高 iPSC 的生成效率,对人胚胎干细胞具有保护作用。

Thiazovivin
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Thiazovivin

Rating icon 很棒
纯度: 98.75%
产品编号 T2155Cas号 1226056-71-8

Thiazovivin 是一种ROCK 抑制剂,能提高 iPSC 的生成效率,对人胚胎干细胞具有保护作用。

规格价格库存数量
1 mg¥ 207现货
2 mg¥ 373现货
5 mg¥ 663现货
10 mg¥ 1,130现货
25 mg¥ 2,270现货
50 mg¥ 3,480现货
100 mg¥ 4,930现货
1 mL x 10 mM (in DMSO)¥ 670现货
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产品介绍

生物活性
产品描述
Thiazovivin, a ROCK inhibitor (IC50: 0.5 μM), increases the survival rate of hESC.
靶点活性
ROCK:~0.5 μM (cell free)
体外活性
当细胞在E-cadherin阻断抗体的存在下培养时,细胞在Thiazovivin处理后形成的大型、紧密的聚集体严重受阻,且观察到广泛的细胞死亡。Thiazovivin(2 μM)抑制ROCK活性,并在与Y-27632(10 μM)相似的水平上保护hESCs,后者是一种广泛使用的选择性ROCK抑制剂[1]。在Thiazovivin处理组中,囊胚和胚胎细胞团在给养细胞上的附着率高于对照组。Thiazovivin处理增加了OCT4和NANOG基因的多能性标记以及粘附分子E-cadherin[2]。通过在重编程培养中添加Thiazovivin,CB细胞的重编程效率增加了10倍以上[3]。
激酶实验
Synthesis of compound immobilized affinity matrixes: Compounds Tzv and its inactive analog (10 mg each) in DMSO (500 μL) and Et3N (10.4 μL) were added to Reacti-Gel (0.5 mL, 25 μmol) that was washed by DMSO in an Eppendorf vial. The reaction mixture was incubated at room temperature until the starting material disappeared (determined by HPLC). After the disappearance of the starting material, ethanolamine (15 μL) was added and the resulting mixture was incubated at room temperature overnight to block the Reacti-Gel. The resulting affinity matrices were washed thoroughly with DMSO (500 μL × 4), PBS (500 μL × 2), and stored at 4 °C in NaN3 solution (0.1% in PBS). Affinity pull-down was performed as described previously. Briefly, whole cell lysates were pretreated with the unfunctionalized affinity matrix at 4 °C for 1.5 h. After washing three times, samples were incubated with the positive or negative affinity matrix at 4 °C for 1 h. After heat shock, samples were loaded and separated on a 4–20% Tris-Glycine SDS PAGE and silver stained with a Silver Stain Plus Kit. The differentially retained protein bands were cut, destained, and analyzed with LCMS [1].
细胞实验
Chemically defined and feeder-free human embryonic stem cell (hESC) culture was described briefly as following. hESCs were grown on Matrigel-coated tissue culture plates in N2B27-CDM [DMEM-F12 supplemented with 1× N2 supplements, 1× B27 supplements, 2 mM L glutamine, 0.11 mM 2-mercaptoethanol, 1× nonessential amino acids, and 0.5 mg/mL BSA (fraction V)] and 20 ng/mL bFGF. Human ESCs were passaged every five to six days with 0.05% trypsin. Murine ESCs are cultured in knockout DMEM supplement with 2 mM L glutamine, 1× nonessential amino acids, 15% serum replacement, and 1 × 10^3 ng/mL leukemia inhibitory factor (LIF). For clonal survival assays, single hESCs were diluted to clonal density and plated onto 96-well Matrigel-coated plate. For low-density survival assays, 500 cells were plated onto 96-well Matrigel-coated plate. To visualize hESC colonies, cultures were fixed in 4% paraformaldehyde in phosphate buffered saline (PBS) for 5 min, washed once in PBS, then stained for alkaline phosphatase (ALP) activity as described in the manufacturer's instructions. ALP-positive colonies were counted on an inverted microscope. For growing hESCs in mouse medium, HES2, HUES7, HUES9, and HUES1-Oct4-GFP were cultured in murine ESC (mESC) growth media supplemented with 1-μM mitogen-activated protein kinase/extracellular signal-regulated kinase kinase (MEK) inhibitor PD0325901 and 5-μM p38 inhibitor SB202190 and 1 × 10^3 human LIF [1].
化学信息
分子量311.36
分子式C15H13N5OS
CAS No.1226056-71-8
SmilesO=C(NCc1ccccc1)c1csc(Nc2ccncn2)n1
密度1.38 g/cm3
储存&溶解度
存储Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
溶解度信息
DMSO: 60 mg/mL (192.7 mM)
溶液配制表
1mg5mg10mg50mg
1 mM3.2117 mL16.0586 mL32.1172 mL160.5858 mL
5 mM0.6423 mL3.2117 mL6.4234 mL32.1172 mL
10 mM0.3212 mL1.6059 mL3.2117 mL16.0586 mL
20 mM0.1606 mL0.8029 mL1.6059 mL8.0293 mL
50 mM0.0642 mL0.3212 mL0.6423 mL3.2117 mL
100 mM0.0321 mL0.1606 mL0.3212 mL1.6059 mL

计算器

  • 摩尔浓度 计算器
  • 稀释 计算器
  • 配液 计算器
  • 分子量 计算器

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法:
TargetMol | Animal experiments比如您的给药剂量是 10 mg/kg ,每只动物体重 20 g ,给药体积 100 μLTargetMol | Animal experiments 一共给药动物 10 只 ,您使用的配方为 5% TargetMol | reagent DMSO+ 30%PEG300+ 5%Tween 80 + 60% ddH2O. 那么您的工作液浓度为 2 mg/mL
母液配置方法: 2 mg 药物溶于 50 μLDMSOTargetMol | reagent ( 母液浓度为 40 mg/mL ), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:50μLDMSOTargetMol | reagent 母液,添加 300 μLPEG300TargetMol | reagent 混匀澄清,再加 50μLTween 80, 混匀澄清,再加 600μLddH2OTargetMol | reagent 混匀澄清

以上为“体内实验配液计算器”的使用方法举例,并不是具体某个化合物的推荐配制方式,请根据您的实验动物和给药方式选择适当的溶解方案。

1 请输入动物实验的基本信息
mg/kg
g
μL
2 请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
% DMSO
%
%Tween 80
%ddH2O

剂量转换

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