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Mdivi-1 (Mitochondrial division inhibitor 1) 是一种线粒体分裂抑制剂,抑制 DRP1 和 Dynamin I (IC50=1-10 μM)。Mdivi-1 可以抑制线粒体自噬。
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Mdivi-1 (Mitochondrial division inhibitor 1) 是一种线粒体分裂抑制剂,抑制 DRP1 和 Dynamin I (IC50=1-10 μM)。Mdivi-1 可以抑制线粒体自噬。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
1 mg | ¥ 153 | 现货 | |
5 mg | ¥ 315 | 现货 | |
10 mg | ¥ 523 | 现货 | |
25 mg | ¥ 987 | 现货 | |
50 mg | ¥ 1,790 | 现货 | |
100 mg | ¥ 2,530 | 现货 | |
200 mg | ¥ 3,730 | 现货 | |
500 mg | ¥ 5,950 | 现货 | |
1 mL x 10 mM (in DMSO) | ¥ 315 | 现货 |
产品描述 | Mdivi-1 (Mitochondrial division inhibitor 1) is a mitochondrial division inhibitor that inhibits DRP1 and Dynamin I (IC50=1-10 μM). Mdivi-1 inhibits mitochondrial autophagy. |
靶点活性 | Dnm1 GTPase:1 μM-10 μM |
体外活性 | 方法:人乳腺癌细胞 MDA-MB-231 用 Mdivi-1 (5-40 μM) 处理 8 h,使用 Immunostaining 方法分析有丝分裂纺锤体的异常。 结果:Mdivi-1 剂量依赖性地增加具有纺锤体异常的有丝分裂细胞的百分比。[1] 方法:人肺癌细胞 H460、A549 和人结直肠癌细胞 HCT116 用 Mdivi-1 (20-50 μM) 处理 24 h,使用 Flow Cytometry 方法检测细胞死亡情况。 结果:Mdivi-1 显著抑制三种肿瘤细胞的死亡。[2] |
体内活性 | 方法:为检测体内活性,将 Mdivi-1 (50 mg/kg) 腹腔注射给 C57BL/6 小鼠,随后通过急性眼压升高诱导短暂的视网膜缺血。 结果:Mdivi-1 治疗阻断了缺血视网膜中的凋亡细胞死亡,并显著提高了缺血后两周视网膜神经节细胞的存活率。[3] 方法:为研究对实验性自身免疫性脑脊髓炎 (EAE) 的影响,将 Mdivi-1 (25 mg/kg) 腹腔注射给诱导 EAE 的 C57BL/6 小鼠,每天一次,持续二十五天。 结果:Mdivi-1 通过减少中枢神经系统中的脱髓鞘和细胞浸润,有效地抑制了 EAE 的严重程度。Mdivi-1 通过调节 Th1/Th17 和调节性 T 细胞之间的平衡,在 EAE 中具有治疗潜力。[4] |
激酶实验 | All GTPase assay reactions are started in a 200 μL volume, of which 150 μL is placed into the well of a 96-well plate. Depletion of NADH, as monitored by reading the A340 of the reaction, is measured every 20 s for a total of 40 min using a SpectraMAX 250 96-well plate reader. Spectrophotometric data are transferred to Excel and the measured steady state depletion of NADH over time is converted to protein activity. |
细胞实验 | Mdivi-1 is dissolved in DMSO. YPGlycerol plates are topped with 10 mL YPGlycerol containing 1% low melt agar and 75 μM mdivi-1, and cells are spotted 12 hours later using a 48 well pinning device. After pinning cells, plates are incubated at 24°C or 37°C and imaged using an Eagle Eye II imaging system. |
别名 | Mitochondrial division inhibitor 1 |
分子量 | 353.22 |
分子式 | C15H10Cl2N2O2S |
CAS No. | 338967-87-6 |
存储 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. | ||||||||||||||||||||||||||||||||||||||||
溶解度信息 | DMSO: 50 mg/mL (141.55 mM) 10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.53 mg/mL (9.99 mM), Suspension. Please add co-solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. | ||||||||||||||||||||||||||||||||||||||||
溶液配制表 | |||||||||||||||||||||||||||||||||||||||||
10% DMSO+40% PEG300+5% Tween 80+45% Saline/DMSO
DMSO
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