Powder: -20°C for 3 years | In solvent: -80°C for 1 year
CFTR(inh)-172 (CFTR Inhibitor-172) 是一种不依赖电压的选择性 CFTR 抑制剂,在2分钟内可逆地抑制CFTR 短路电流的Ki 值为300 nM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 236 | 现货 | ||
2 mg | ¥ 329 | 现货 | ||
5 mg | ¥ 593 | 现货 | ||
10 mg | ¥ 897 | 现货 | ||
25 mg | ¥ 1,620 | 现货 | ||
50 mg | ¥ 2,820 | 现货 | ||
100 mg | ¥ 4,190 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 631 | 现货 |
产品描述 | CFTR(inh)-172 (CFTR Inhibitor-172) is a voltage-independent, selective CFTR inhibitor. |
靶点活性 | CFTR:300 nM(Ki) |
体外活性 | 6小时20 μg CFTRinh-172完全消除霍乱弧菌诱导的肠液分泌而不影响体内霍乱弧菌的生长. |
体内活性 | CFTRinh-172作为一种选择性的CFTR通道抑制剂,能够完全消除兔泪腺兔腺泡和导管细胞中的Cl-电流。CFTRinh-172时间和剂量依赖性的抑制了CFTR介导的碘离子转运,并且有效抑制多种激动剂或激活剂的CFTR激活。CFTRinh-172不依赖CFTR抑制而诱导ROS产生,线粒体衰竭和NF-κB信号传导途径的激活。 |
激酶实验 | Screening procedures: Assays are done using a customized screening system consisting of a 3-meter robotic arm, CO2 incubator, plate washer, liquid-handling workstation, bar code reader, delidding station, and two FLUOstar fluorescence platereaders, each equipped with two syringe pumps and HQ500/20X (500 ± 10 nm) excitation and HQ535/30M (535 ± 15 nm) emission filters. The robotic system is integrated using SAMI version 3.3 software modified for two platereaders. Custom software is written in Microsoft VBA (Visual Basic for Applications) to compute base-line–subtracted, normalized fluorescence slopes (giving halide influx rates) from stored data files. The assay is set up by loading the incubator (37°C, 90% humidity, 5% CO2) with 40–60 96-well plates containing the FRT cells, and loading a carousel with 96-well plates containing test compounds and disposable plastic pipette tips. To initiate the assay, each well of a 96-well plate is washed three times in PBS (300 μl/wash), leaving 50 μL PBS. Ten microliters of a CFTR-activating cocktail (5 μM forskolin, 100 μM IBMX, 25 μM apigenin in PBS) is added, and after 5 minutes one test compound (0.5 μL of 1 mM DMSO solution) is added to each well to give 10 μM final concentration. After 10 minutes, 96-well plates are transferred to a platereader for fluorescence assay. Each well is assayed individually for CFTR-mediated I– transport by recording fluorescence continuously (200 ms per point) for 2 seconds (base line) and then for 12 seconds after rapid (<0.5 seconds) addition of 165 μL of isosmolar PBS in which 137 mM Cl– was replaced by I–. |
细胞实验 | Cell toxicity is assayed by the dihydrorhodamine method at 24 hours after cell incubation with 0–1,000 μM inhibitor. (Only for Reference) |
别名 | CFTRinh 172, CFTR Inhibitor-172, CFTRinh-172, CFTRinh172 |
分子量 | 409.4 |
分子式 | C18H10F3NO3S2 |
CAS No. | 307510-92-5 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 40.9 mg/mL (100 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.4426 mL | 12.213 mL | 24.426 mL | 61.065 mL |
5 mM | 0.4885 mL | 2.4426 mL | 4.8852 mL | 12.213 mL | |
10 mM | 0.2443 mL | 1.2213 mL | 2.4426 mL | 6.1065 mL | |
20 mM | 0.1221 mL | 0.6106 mL | 1.2213 mL | 3.0532 mL | |
50 mM | 0.0489 mL | 0.2443 mL | 0.4885 mL | 1.2213 mL | |
100 mM | 0.0244 mL | 0.1221 mL | 0.2443 mL | 0.6106 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
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