N-Methyl-D-aspartic acid is an amino acid that, as the D-isomer, is the defining agonist for the NMDA (N-Methyl-D-aspartic acid) receptor subtype of glutamate receptors.

NMDA是一种兴奋性氨基酸神经递质，仅与NMDA受体结合，不影响其他谷氨酸受体（例如AMPA和kainate受体）。NMDA特异性结合于NMDA受体的NR2亚单位，随后促进非特异性阳离子通道的打开，允许Ca2+和Na+进入细胞内，以及K+离开细胞。NMDA受体的激活能够产生兴奋性突触后电位（EPSP），并触发细胞内Ca2+含量的增加，进一步参与多种信号传递途径。NMDA受体在广泛的生理（例如长期增强和神经可塑性）及病理过程（例如兴奋毒性和癫痫）中发挥关键作用。[1]

微注射NMDA（0.2 nM）对MF、IF、IL和EL分别具有显著影响，降低了交尾频次和插入频次，并缩短了插入和射精潜伏期。分别在交配测试30分钟内，NMDA和AP-5显著地促进和抑制了射精行为。向PVN双侧微注射NMDA显著增加了基线LSNA，LSNA峰值增量在NMDA注入PVN后5分钟内出现[3]。

Adrenal membranous homogenate suspensions are incubated with 10 nM [3H]Glu in 500/zl 50 mM Tris-acetate buffer (pH 7.4) at 2°C or 30°C in the presence and absence of various compounds. Incubation is terminated by the addition of 3 mL ice-cold buffer and subsequent filtration through a Whatman GF/B glass filter under a constant vacuum of 15 mm Hg. After washing the filter 4 times with 3 mL icecold buffer, the radioactivity trapped on the filter is measured by a liquid scintillation spectrometer using 5 mL modified Triton-toluene scintillant at a counting efficiency of 40-42%. The radioactivity found in the presence of 1 mM non-radioactive Glu is subtracted from each experimental value to obtain the specific binding of [3H]GIu in accordance with the y-aminobutyric acid (GABA) receptor binding assay system. The kinetic parameters of [3H]GIu binding, Kd and Bma x, are calculated by Scatchard analysis of the specific binding using a personal computer with a programme for non-linear regression analysis developed in our own laboratory.