Deoxycholic acid sodium salt (Sodium deoxycholate) can activate the G protein-coupled bile acid receptor TGR5 that stimulates brown adipose tissue (BAT) thermogenic activity.

Deoxycholic acid(DCA)和鹅Deoxycholic acid(CDCA)是十二指肠反流的常见成分，在许多生理和病理过程中协同作用。细胞连续暴露于100 μM的CDCA和Deoxycholic acid，在pH 5.5条件下最长达120分钟，以模拟体外慢性局部复发性疾病。胃癌细胞系MGC803在含有100 μMDeoxycholic acid和CDCA的酸化培养基(pH 5.5)中暴露，以进行研究。作为对照，生成一个未处理的、处于对数生长期的MGC803细胞系，在正常pH培养基中使用。经过每天10分钟暴露于酸化的胆酸后，经过60周，MGC803抗性细胞能够在120分钟的暴露后存活和增殖[2]。

MGC803 cells are cultured in Roswell Park Memorial Institute media supplemented with 10% fetal calf serum and 100 U/mL Penicillin and 100 mg/mL Streptomycin. To generate MGC803-resistant cells, the pH value of the MGC803 culture medium is adjusted to the experimental conditions using the hydrochloric acid (A). The bile acids GCDA and Deoxycholic acid are diluted to optimal working concentrations of 100 μM (B) with culture medium, and the overall pH (A+B) is adjusted to pH 5.5, simulating the gastric environment. Initially, MGC803 cells are chronically exposed to acidified medium with bile acids (A+B) for 10 min every 24 h. The experimental time and conditions are optimized in our preliminary experiments, which show that 10 min is enough and does not result in cell damage. This procedure is repeated and it takes 60 weeks for the MGC803 cells to survive and proliferate under the exposure of A+B for 120 min. Control untreated cells are cultured in neutral RPMI medium at pH 7.4 in parallel to the resistant cells for 60 weeks. The morphological changes in MGC803 cells exposed to acidified bile acids (A+B) are documented at 30 and 60 weeks[2].