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AUT1

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产品编号 T5183Cas号 1311136-84-1

AUT1 是 Kv3 钾离子通道调节剂,对人重组 Kv3.1b 和 Kv3.2a 的pEC50值分别为 5.33 和 5.31,对其活性是对人重组 Kv3.3 通道的 10 倍,pEC50值为 4.5。

AUT1
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AUT1

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纯度: 99.80%
产品编号 T5183Cas号 1311136-84-1

AUT1 是 Kv3 钾离子通道调节剂,对人重组 Kv3.1b 和 Kv3.2a 的pEC50值分别为 5.33 和 5.31,对其活性是对人重组 Kv3.3 通道的 10 倍,pEC50值为 4.5。

规格价格库存数量
1 mg
¥ 322
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5 mg
¥ 787
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10 mg
¥ 1,180
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25 mg
¥ 2,080
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50 mg
¥ 3,190
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100 mg
¥ 4,620
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1 mL x 10 mM (in DMSO)
¥ 588
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产品介绍

生物活性
产品描述
AUT1 (AUT-1) is a novel specific modulator of Kv3 channels (EC50: 4.7 and 4.9 uM for Kv3.1b and Kv3.2a).
靶点活性
Kv3.2a:4.9 μM, Kv3.1b:4.7 μM
体外活性
AUT1增强了人类重组Kv3.1b和Kv3.2a通道介导的电流。对于Kv3.1b和Kv3.2a,AUT1的pEC50值分别为5.33(4.7 μM)和5.31(4.9 μM)[1]。使用稳定表达大鼠Kv3.1通道的中国仓鼠卵巢细胞,较低浓度的AUT1可将Kv3.1电流的激活电压向负电位移动,从典型神经元静息电位引起的去极化所诱发的电流增加[2]。
细胞实验
The population patch-clamp mode of an automated voltage clamp recording with IonWorks Quattro was used. Briefly, the effects on these channels were tested using 384-well population patch-clamp plates. Seal resistance was measured for each well, and cells were perforated by incubation with 100 mg/ml amphotericin B. Cells were held at 270 mV and stepped to 215 mV for 100 milliseconds (partial channel activation), and after 100 milliseconds at 270 mV, a second pulse to 140 mV was applied for 50 milliseconds (full channel activation). In all of the experiments, this voltage protocol was applied to cells before and following a 3-minute incubation with AUT1. 1-Cyclohexyl-1-[(7,8-dimethyl-2-oxo-1H-quinolin-3-yl)methyl]-3-phenylurea (10 mM), which we had previously found to be a potent and full activator of human Kv3.1 and Kv3.2 channels, was included in all assays as a standard. An external buffer with the addition of dimethyl sulfoxide (DMSO) was also tested to provide a vehicle baseline. Recordings were performed in the following buffers: Dulbecco's phosphate-buffered saline (PBS) with MgCl2 and CaCl2 as an extracellular solution and 50 mM KCl, 100 mM K-gluconate, 3.2 mM MgCl2, and 5 mM HEPES, pH 7.3 adjusted with KOH, as an intracellular solution. An online correction of 115–20 mV was applied to correct for junction potentials. The current signal was sampled at 10 kHz [1].
化学信息
分子量341.36
分子式C18H19N3O4
CAS No.1311136-84-1
SmilesCC[C@H]1NC(=O)N(C1=O)c1ccc(Oc2ccc(C)c(OC)c2)nc1
密度no data available
储存&溶解度
存储Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
溶解度信息
DMSO: 250 mg/mL (732.36 mM), Sonication is recommended.
溶液配制表
DMSO
1mg5mg10mg50mg
1 mM2.9295 mL14.6473 mL29.2946 mL146.4729 mL
5 mM0.5859 mL2.9295 mL5.8589 mL29.2946 mL
10 mM0.2929 mL1.4647 mL2.9295 mL14.6473 mL
20 mM0.1465 mL0.7324 mL1.4647 mL7.3236 mL
50 mM0.0586 mL0.2929 mL0.5859 mL2.9295 mL
100 mM0.0293 mL0.1465 mL0.2929 mL1.4647 mL

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计算器

  • 摩尔浓度 计算器
  • 稀释 计算器
  • 配液 计算器
  • 分子量 计算器

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法:
TargetMol | Animal experiments比如您的给药剂量是 10 mg/kg ,每只动物体重 20 g ,给药体积 100 μLTargetMol | Animal experiments 一共给药动物 10 只 ,您使用的配方为 5% TargetMol | reagent DMSO+ 30%PEG300+ 5%Tween 80 + 60% ddH2O. 那么您的工作液浓度为 2 mg/mL
母液配置方法: 2 mg 药物溶于 50 μLDMSOTargetMol | reagent ( 母液浓度为 40 mg/mL ), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:50μLDMSOTargetMol | reagent 母液,添加 300 μLPEG300TargetMol | reagent 混匀澄清,再加 50μLTween 80, 混匀澄清,再加 600μLddH2OTargetMol | reagent 混匀澄清

以上为“体内实验配液计算器”的使用方法举例,并不是具体某个化合物的推荐配制方式,请根据您的实验动物和给药方式选择适当的溶解方案。

1 请输入动物实验的基本信息
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2 请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
% DMSO
%
%Tween 80
%ddH2O

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