N-Formyl-Met-Leu-Phe (N-Formyl-MLF) (fMLF) is a synthetic peptide that acts as an agonist at formyl peptide receptors (FPR; Ki = 0.8 pM).

N-Formyl-Met-Leu-Phe 受体与 Ni GTP 调节蛋白相关联，N-Formyl-Met-Leu-Phe 复合物在控制多个中性粒细胞功能方面具有重要作用[1]。

N-Formyl-Met-Leu-Phe(fMLP)在斑马鱼和兔子中促进了骨骼形成，表明其在体内具有生理学相关性[2]。

ebrafish (Danio rerio) embryos were collected from spontaneous spawnings reared at 28.5 ℃. Three days postfertilization (～1 day after hatching), feeding of the commercial powder for baby fish was started. Zebrafish embryos were stimulated with fMLP from 8 to 48 h postfertilization (hpf), washed, and raised at 28.5 ℃. At 5 days postfertilization (dpf), 20 zebrafish were stained with Alizarin Red for examination of the effect of fMLP on bone formation. Zebrafish were fixed in 4% paraformaldehyde for 12–18 h. Following fixation, fish were depigmented overnight in 1% KOH containing 3% H2O2 and immersed for 2 h in 0.5% KOH containing 0.05% Alizarin Red, pH 4.2. Alizarin-stained fish were then cleared again in 1% KOH and observed under a microscope[2].